Marked stimulation of cell adhesion and motility by ladsin, a laminin-like scatter factor.

Marked stimulation of cell adhesion and motility by ladsin, a laminin-like scatter factor. Research Abstract Details 

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Marked stimulation of cell adhesion and motility by ladsin, a laminin-like scatter factor. Abstract Text:

Y Kikkawa,M Umeda,K Miyazaki,

Ladsin is a large cell-adhesive protein with potent cell-scattering activity, which was recently identified in the culture of a malignant human gastric carcinoma cell line [Miyazaki, K. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 11767-11771]. It is a heterotrimeric protein, containing a 140-kDa subunit similar or identical to the laminin B2t chain. Ladsin is similar to the keratinocyte-derived matrix proteins, "epiligrin" and "kalinin." In the present study, the cell-adhesion and cell-migration activities of ladsin were examined in comparison with those of three cell adhesion proteins, laminin, fibronectin, and vitronectin. Ladsin showed high cell-adhesion activity toward rat liver cell line BRL at concentrations 4-20-times lower than in the case of the other three proteins. In a monolayer culture, ladsin stimulated the migration of BRL cells about 2-times more strongly than the others, as compared at the minimal concentrations required for the maximal cell-adhesion activity. In Boyden chambers, ladsin stimulated both the chemotactic and chemokinetic migration of BRL cells. When the effect of anti-integrin antibodies on the adhesion of human fibrosarcoma cell line HT1080 was examined, the adhesion to ladsin was effectively inhibited by both the anti-integrin alpha 3 and beta 1 antibodies, but not the anti-integrin alpha 6 antibody, indicating that the primary receptor of ladsin is integrin alpha 3 beta 1. These results demonstrate that ladsin is a unique extracellular matrix component which may play a major role in cell migration.

Marked stimulation of cell adhesion and motility by ladsin, a laminin-like scatter factor. Publishing Authors By Initials

Y Kikkawa,M Umeda,K Miyazaki,

For similar proteins: blood proteins: vitronectin research abstracts see: proteins: blood proteins: vitronectin research

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Marked stimulation of cell adhesion and motility by ladsin, a laminin-like scatter factor. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 116

Page Numbers: 862-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1994

Marked stimulation of cell adhesion and motility by ladsin, a laminin-like scatter factor. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Marked stimulation of cell adhesion and motility by ladsin, a laminin-like scatter factor. Keywords Mesh Terms:

KEYWORDS: Vitronectin

MESH TERMS: drug effects

Chemical & Substance for Abstract: Marked stimulation of cell adhesion and motility by ladsin, a laminin-like scatter factor. Information

Substance Name: Hepatocyte Growth Factor

Registry Number: 67256-21-7

Grant and Affiliation Information for Marked stimulation of cell adhesion and motility by ladsin, a laminin-like scatter factor.

AFFILIATION: Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University.

Country: JAPAN

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MEDLINETA: J Biochem

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