Mapping the anthocyaninless (anl) locus in rapid-cycling Brassica rapa (RBr) to linkage group R9.

Mapping the anthocyaninless (anl) locus in rapid-cycling Brassica rapa (RBr) to linkage group R9. Research Abstract Details 

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Mapping the anthocyaninless (anl) locus in rapid-cycling Brassica rapa (RBr) to linkage group R9. Abstract Text:

Carrie Burdzinski,Douglas L Wendell,Carrie Burdzinski,Douglas L Wendell,Carrie Burdzinski,Douglas L Wendell,

BACKGROUND: Anthocyanins are flavonoid pigments that are responsible for purple coloration in the stems and leaves of a variety of plant species. Anthocyaninless (anl) mutants of Brassica rapa fail to produce anthocyanin pigments. In rapid-cycling Brassica rapa, also known as Wisconsin Fast Plants, the anthocyaninless trait, also called non-purple stem, is widely used as a model recessive trait for teaching genetics. Although anthocyanin genes have been mapped in other plants such as Arabidopsis thaliana, the anl locus has not been mapped in any Brassica species. RESULTS: We tested primer pairs known to amplify microsatellites in Brassicas and identified 37 that amplified a product in rapid-cycling Brassica rapa. We then developed three-generation pedigrees to assess linkage between the microsatellite markers and anl. 22 of the markers that we tested were polymorphic in our crosses. Based on 177 F2 offspring, we identified three markers linked to anl with LOD scores >or= 5.0, forming a linkage group spanning 46.9 cM. Because one of these markers has been assigned to a known B. rapa linkage group, we can now assign the anl locus to B. rapa linkage group R9. CONCLUSION: This study is the first to identify the chromosomal location of an anthocyanin pigment gene among the Brassicas. It also connects a classical mutant frequently used in genetics education with molecular markers and a known chromosomal location.

Mapping the anthocyaninless (anl) locus in rapid-cycling Brassica rapa (RBr) to linkage group R9. Publishing Authors By Initials

C Burdzinski,DL Wendell,C Burdzinski,DL Wendell,C Burdzinski,DL Wendell,

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Mapping the anthocyaninless (anl) locus in rapid-cycling Brassica rapa (RBr) to linkage group R9. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: BMC genetics

VOLUME: 8

Page Numbers: 64

Journal Abbreviation: BMC Genet.

ISSN: 1471-2156

DAY: 25

MONTH: 09

YEAR: 2007

Mapping the anthocyaninless (anl) locus in rapid-cycling Brassica rapa (RBr) to linkage group R9. Information

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LANGUAGE: eng

NlmUniqueID: 100966978

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Grant and Affiliation Information for Mapping the anthocyaninless (anl) locus in rapid-cycling Brassica rapa (RBr) to linkage group R9.

AFFILIATION: Department of Biological Sciences, Oakland University, 2200 N, Squirrel Rd, Rochester, MI 48309-4401, USA. ceburdz2@oakland.edu

Country: England

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