Mapping ATP-binding cassette transporter gene expression profiles in melanocytes and melanoma cells.

Mapping ATP-binding cassette transporter gene expression profiles in melanocytes and melanoma cells. Research Abstract Details 

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Mapping ATP-binding cassette transporter gene expression profiles in melanocytes and melanoma cells. Abstract Text:

Susanne Heimerl,Anja K Bosserhoff,Thomas Langmann,Josef Ecker,Gerd Schmitz,

ATP-binding cassette (ABC) transporters regulate the transport of a variety of physiologic substrates. Moreover, several human ABC proteins are responsible for drug exclusion in compound-treated tumor cells, providing cellular mechanisms for the development of multidrug resistance and, therefore, playing an important role in malignant transformation. As only limited information exists on the role of ABC transporters in melanoma, the aim of the study was to generate a complete expression profile of ABC transporters in this tumor entity. Using a TaqMan low-density array for 47 human ABC transporters, mRNA expression analysis was performed from normal human epidermal melanocytes (NHEM P2 and NHEM P3), nine different cell lines originating from primary melanoma (Mel Ei, Mel Juso, Mel Ho and Mel Wei), and metastases of malignant melanoma (Mel Im, Mel Ju, SK Mel 28, HTZ 19 and HMB2). Cell line-specific expression levels were compared with gene expression in pooled RNA from a variety of other human tissues. High expression levels were detected in pooled tissue RNA as well as in cells of melanocytic origin for ABCA5, ABCB2, ABCB6, ABCD3, ABCD4, ABCF1, ABCF2 and ABCF3, whereas ABCB5 revealed a melanocyte-specific high transcript level. In relation to normal melanocytes, ABCB3, ABCB6, ABCC2, ABCC4, ABCE1 and ABCF2 were significantly increased in melanoma cell lines, whereas ABCA7, ABCA12, ABCB2, ABCB4, ABCB5 and ABCD1 showed lower expression levels. In summary, we present here for the first time an ABC-transporter mRNA expression profile in melanoma in comparison to normal melanocytes. The differentially regulated ABC transporters detected by our approach may be candidate genes involved in melanoma tumorigenesis, progression and therapy resistance and could therefore be of great importance to identify novel options for melanoma therapy.

Mapping ATP-binding cassette transporter gene expression profiles in melanocytes and melanoma cells. Publishing Authors By Initials

S Heimerl,AK Bosserhoff,T Langmann,J Ecker,G Schmitz,

For similar biological factors: biological markers: tumor markers, biological research abstracts see: biological factors: biological markers: tumor markers, biological research

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Mapping ATP-binding cassette transporter gene expression profiles in melanocytes and melanoma cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Melanoma research

VOLUME: 17

Page Numbers: 265-73

Journal Abbreviation: Melanoma Res.

ISSN: 0960-8931

DAY: 19

MONTH: Oct

YEAR: 2007

Mapping ATP-binding cassette transporter gene expression profiles in melanocytes and melanoma cells. Information

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LANGUAGE: eng

NlmUniqueID: 9109623

Mapping ATP-binding cassette transporter gene expression profiles in melanocytes and melanoma cells. Keywords Mesh Terms:

KEYWORDS: Tumor Markers, Biological

MESH TERMS: genetics

Chemical & Substance for Abstract: Mapping ATP-binding cassette transporter gene expression profiles in melanocytes and melanoma cells. Information

Substance Name: Tumor Markers, Biological

Registry Number: 0

Grant and Affiliation Information for Mapping ATP-binding cassette transporter gene expression profiles in melanocytes and melanoma cells.

AFFILIATION: Institute of Clinical Chemistry, University of Regensburg, Regensburg, Germany.

Country: England

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MEDLINETA: Melanoma Res

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