Mammalian lin-7 stabilizes polarity protein complexes.

Mammalian lin-7 stabilizes polarity protein complexes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Mammalian lin-7 stabilizes polarity protein complexes. Abstract Text:

Samuel W Straight,Jay N Pieczynski,Eileen L Whiteman,Chia-Jen Liu,Ben Margolis,

Mammalian Lin-7 forms a complex with several proteins, including PALS1, that have a role in polarity determination in epithelial cells. In this study we have found that loss of Lin-7 protein from the polarized epithelial cell line Madin-Darby canine kidney II by small hairpin RNA results in defects in tight junction formation as indicated by lowered transepithelial electrical resistance and mislocalization of the tight junction protein ZO-1 after calcium switch. The knock down of Lin-7 also resulted in the loss of expression of several Lin-7 binding partners, including PALS1 and the polarity protein PATJ. The effects of Lin-7 knock down were rescued by the exogenous expression of murine Lin-7 constructs that contained the L27 domain, but not the PDZ domain alone. Furthermore, exogenously expressed PALS1, but not other Lin-7 binding partners, also rescued the effects of Lin-7 knock down, including the restoration of PATJ protein in rescued cell lines. Finally, the effects of Lin-7 knock down appeared to be due to instability of PALS1 protein in the absence of Lin-7, as indicated by an increased rate of PALS1 protein degradation. Taken together, these results indicate that Lin-7 functions in tight junction formation by stabilizing its membrane-associated guanylate kinase binding partner PALS1.

Mammalian lin-7 stabilizes polarity protein complexes. Publishing Authors By Initials

SW Straight,JN Pieczynski,EL Whiteman,CJ Liu,B Margolis,

For similar cells: cellular structures: cell membrane: cell membrane structures: intercellular junctions: tight junctions research abstracts see: cells: cellular structures: cell membrane: cell membrane structures: intercellular junctions: tight junctions research

PUBMED ID PMID:

MEDLINE DATE:

Mammalian lin-7 stabilizes polarity protein complexes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 281

Page Numbers: 37738-47

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 1

MONTH: 10

YEAR: 2006

Mammalian lin-7 stabilizes polarity protein complexes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Mammalian lin-7 stabilizes polarity protein complexes. Keywords Mesh Terms:

KEYWORDS: Tight Junctions

MESH TERMS: metabolism

Chemical & Substance for Abstract: Mammalian lin-7 stabilizes polarity protein complexes. Information

Substance Name: RNA

Registry Number: 63231-63-0

Grant and Affiliation Information for Mammalian lin-7 stabilizes polarity protein complexes.

AFFILIATION: Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK58208

ACRONYM: DK

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Mammalian lin-7 stabilizes polarity protein complexes Related Publications

• "Steel wool keratopathy": a clinical sign of chronic inflammation.
• A novel Crumbs3 isoform regulates cell division and ciliogenesis via importin beta interactions.
• A novel trinucleotide repeat expansion at chromosome 3q26.2 identified by a CAG/CTG repeat expansion detection array.
• Apical junctional complexes and cell polarity.
• Bex1 knock out mice show altered skeletal muscle regeneration.
• Body dysmorphic disorder symptoms among patients with acne vulgaris.
• Confronting proviral HIV infection.
• Digital imaging of wounds: are measurements reproducible among observers?
• Effect of fluoride on artificial caries lesion progression and repair in human enamel: regulation of mineral deposition and dissolution under in vivo-like conditions.
• Emergency medical services education: past, present, and future.
• Expression of an intron-containing beta-tubulin mRNA in catfish olfactory epithelium.
• Hepatitis B vaccination of men who have sex with men attending an urban STD clinic: impact of an ongoing vaccination program, 1998-2003.
• Huntington's disease--like 2 is associated with CUG repeat-containing RNA foci.
• Interruption of antiretroviral treatment in HIV-infected patients with preserved immune function is associated with a low rate of clinical progression: a prospective study by AIDS Clinical Trials Group 5170.
• Mammalian lin-7 stabilizes polarity protein complexes.
• Mitosis persists in the absence of Cdk1 activity when proteolysis or protein phosphatase activity is suppressed.
• Nuclear receptors and bone.
• Obituary: arun fotedar.
• Olfactory marker protein modulates the cAMP kinetics of the odour-induced response in cilia of mouse olfactory receptor neurons.
• PALS1 regulates E-cadherin trafficking in mammalian epithelial cells.
• Radiation therapy for cutaneous squamous cell carcinoma involving the parotid area lymph nodes: dose and volume considerations.
• Repeat expansion and autosomal dominant neurodegenerative disorders: consensus and controversy.
• Restricted-carbohydrate diets in patients with type 2 diabetes: a meta-analysis.
• Sensate scaffolds coupled to telemetry can monitor in vivo loading from within a joint over extended periods of time.
• Sodium/calcium exchanger expression in the mouse and rat olfactory systems.
• Spindle cell lipoma of the mediastinum: a differential consideration for liposarcoma.
• The prevalence of atopic triad in children with physician-confirmed atopic dermatitis.
• Upper respiratory tract infection in household contacts of acne patients.
• c-Myc and Sp1 contribute to proviral latency by recruiting histone deacetylase 1 to the human immunodeficiency virus type 1 promoter.
• pH triggered self-assembly of native and recombinant amelogenins under physiological pH and temperature in vitro.