Mammalian GPI-anchored proteins require p24 proteins for their efficient transport from the ER to the plasma membrane.

Mammalian GPI-anchored proteins require p24 proteins for their efficient transport from the ER to the plasma membrane. Research Abstract Details 

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Mammalian GPI-anchored proteins require p24 proteins for their efficient transport from the ER to the plasma membrane. Abstract Text:

Satoshi Takida,Yusuke Maeda,Taroh Kinoshita,Satoshi Takida,Yusuke Maeda,Taroh Kinoshita,Satoshi Takida,Yusuke Maeda,Taroh Kinoshita,

The GPI (glycosylphosphatidylinositol) moiety is attached to newly synthesized proteins in the lumen of the ER (endoplasmic reticulum). The modified proteins are then directed to the PM (plasma membrane). Less well understood is how nascent mammalian GPI-anchored proteins are targeted from the ER to the PM. In the present study, we investigated mechanisms underlying membrane trafficking of the GPI-anchored proteins, focusing on the early secretory pathway. We first established a cell line that stably expresses inducible temperature-sensitive GPI-fused proteins as a reporter and examined roles of transport-vesicle constituents called p24 proteins in the traffic of the GPI-anchored proteins. We selectively suppressed one of the p24 proteins, namely p23, employing RNAi (RNA interference) techniques. The suppression resulted in pronounced delays of PM expression of the GPI-fused reporter proteins. Furthermore, maturation of DAF (decay-accelerating factor), one of the GPI-anchored proteins in mammals, was slowed by the suppression of p23, indicating delayed trafficking of DAF from the ER to the Golgi. Trafficking of non-GPI-linked cargo proteins was barely affected by p23 knockdown. This is the first to demonstrate direct evidence for the transport of mammalian GPI-anchored proteins being mediated by p24 proteins.

Mammalian GPI-anchored proteins require p24 proteins for their efficient transport from the ER to the plasma membrane. Publishing Authors By Initials

S Takida,Y Maeda,T Kinoshita,S Takida,Y Maeda,T Kinoshita,S Takida,Y Maeda,T Kinoshita,

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Mammalian GPI-anchored proteins require p24 proteins for their efficient transport from the ER to the plasma membrane. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Biochemical journal

VOLUME: 409

Page Numbers: 555-62

Journal Abbreviation: Biochem. J.

ISSN: 1470-8728

DAY: 15

MONTH: Jan

YEAR: 2008

Mammalian GPI-anchored proteins require p24 proteins for their efficient transport from the ER to the plasma membrane. Information

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LANGUAGE: eng

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AFFILIATION: Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan, and Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan.

Country: England

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MEDLINETA: Biochem J

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