Mammalian 2',3' cyclic nucleotide phosphodiesterase (CNP) can function as a tRNA splicing enzyme in vivo.

Mammalian 2',3' cyclic nucleotide phosphodiesterase (CNP) can function as a tRNA splicing enzyme in vivo. Research Abstract Details 

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Mammalian 2',3' cyclic nucleotide phosphodiesterase (CNP) can function as a tRNA splicing enzyme in vivo. Abstract Text:

Beate Schwer,Anna Aronova,Alejandro Ramirez,Peter Braun,Stewart Shuman,Beate Schwer,Anna Aronova,Alejandro Ramirez,Peter Braun,Stewart Shuman,

Yeast and plant tRNA splicing entails discrete healing and sealing steps catalyzed by a tRNA ligase that converts the 2',3' cyclic phosphate and 5'-OH termini of the broken tRNA exons to 3'-OH/2'-PO(4) and 5'-PO(4) ends, respectively, then joins the ends to yield a 2'-PO(4), 3'-5' phosphodiester splice junction. The junction 2'-PO(4) is removed by a tRNA phosphotransferase, Tpt1. Animal cells have two potential tRNA repair pathways: a yeast-like system plus a distinctive mechanism, also present in archaea, in which the 2',3' cyclic phosphate and 5'-OH termini are ligated directly. Here we report that a mammalian 2',3' cyclic nucleotide phosphodiesterase (CNP) can perform the essential 3' end-healing steps of tRNA splicing in yeast and thereby complement growth of strains bearing lethal or temperature-sensitive mutations in the tRNA ligase 3' end-healing domain. Although this is the first evidence of an RNA processing function in vivo for the mammalian CNP protein, it seems unlikely that the yeast-like pathway is responsible for animal tRNA splicing, insofar as neither CNP nor Tpt1 is essential in mice.

Mammalian 2',3' cyclic nucleotide phosphodiesterase (CNP) can function as a tRNA splicing enzyme in vivo. Publishing Authors By Initials

B Schwer,A Aronova,A Ramirez,P Braun,S Shuman,B Schwer,A Aronova,A Ramirez,P Braun,S Shuman,

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Mammalian 2',3' cyclic nucleotide phosphodiesterase (CNP) can function as a tRNA splicing enzyme in vivo. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: RNA (New York, N.Y.)

VOLUME: 14

Page Numbers: 204-10

Journal Abbreviation: RNA

ISSN: 1469-9001

DAY: 19

MONTH: 12

YEAR: 2007

Mammalian 2',3' cyclic nucleotide phosphodiesterase (CNP) can function as a tRNA splicing enzyme in vivo. Information

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LANGUAGE: eng

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