Male-specific band in random amplified microsatellite polymorphism fingerprints of Holstein cattle.

Male-specific band in random amplified microsatellite polymorphism fingerprints of Holstein cattle. Research Abstract Details 

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Male-specific band in random amplified microsatellite polymorphism fingerprints of Holstein cattle. Abstract Text:

A total of 65 blood samples collected from Holstein cattle were employed for DNA extraction. Genomic DNA were amplified by means of random amplified polymorphic DNA (RAPD). One hundred and one random primers (Operon kits OPAA, OPAO, OPAV, OPC, OPE and OPA-06) were used for polymerase chain reactions (PCR). The PCR products were size fractionated by means of electrophoresis in agarose gel, transferred to a nylon membrane, and then hybridized with a alpha-32P-labeled microsatellite probe for random amplified microsatellite polymorphism (RAMPO) analysis. All the primers produced many amplification patterns in RAMPO fingerprints, but only OPE-01 (5'-CCCAAGGTCC-3') yielded a male-specific strong signal. Among the 65 cases, the specific band could be observed in the RAMPO fingerprints of the 24 male samples but not in the 41 female samples. Therefore, this specific band is peculiar to male cattle.

Male-specific band in random amplified microsatellite polymorphism fingerprints of Holstein cattle. Publishing Authors By Initials

For similar genetic processes: sex determination (genetics) research abstracts see: genetic processes: sex determination (genetics) research

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Male-specific band in random amplified microsatellite polymorphism fingerprints of Holstein cattle. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Proceedings of the National Science Council, Repub

VOLUME: 24

Page Numbers: 41-6

Journal Abbreviation: Proc. Natl. Sci. Counc. Repub.

ISSN: 0255-6596

DAY: 26

MONTH: Jan

YEAR: 2000

Male-specific band in random amplified microsatellite polymorphism fingerprints of Holstein cattle. Information

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LANGUAGE: eng

NlmUniqueID: 8502426

Male-specific band in random amplified microsatellite polymorphism fingerprints of Holstein cattle. Keywords Mesh Terms:

KEYWORDS: Sex Determination (Genetics)

MESH TERMS: methods

Chemical & Substance for Abstract: Male-specific band in random amplified microsatellite polymorphism fingerprints of Holstein cattle. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Male-specific band in random amplified microsatellite polymorphism fingerprints of Holstein cattle.

AFFILIATION: Department of Animal Science and Technology, National Chia-Yi Institute of Technology, Taiwan, ROC.

Country: CHINA (REPUBLIC: 1949- )

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MEDLINETA: Proc Natl Sci Counc Repub Chin

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