Magnesium ion-induced changes in the binding mode of adenylates to chloroplast coupling factor 1.

Magnesium ion-induced changes in the binding mode of adenylates to chloroplast coupling factor 1. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Magnesium ion-induced changes in the binding mode of adenylates to chloroplast coupling factor 1. Abstract Text:

M Higashida,Y Mukohata,

The effect of Mg2+ on the binding of adenylates to isolated chloroplast coupling factor 1 (CF1) was studied using CD spectrometry and ultrafiltration. At adenylate concentrations smaller than 100 muM, one mole of CF1 binds three moles of ATP (or ADP) regardless of the presence of Mg2+. In the presence of Mg2+, the first two ATP's bind to CF1 independently with the same binding constant of 2.5 X 10(-1) muM-1, then the third ATP binds with a much higher affinity of 10 muM-1. In the absence of Mg2+, the first ATP binds to CF1 with a binding constant of 2.5 X 10(-1) muM-1 then the other two ATP's bind less easily with the same binding constant of 4.0 X 10(-2) muM-1. The binding mode of ADP to CF1 is quite similar to that of ATP. In the presence of Mg2+, the binding constants of the first two ADP's are both 7.6 X 10(-2) muM-1, that of the third ADP being 4.0 muM-1. In the absence of Mg2+, the binding constant of the first ADP is 7.6 X 10(-2) muM-1, the constants of the other two ADP's both being 4.0 X 10(-2) muM-1. AMP caused a negligible change in CD.

Magnesium ion-induced changes in the binding mode of adenylates to chloroplast coupling factor 1. Publishing Authors By Initials

M Higashida,Y Mukohata,

For similar proteins: plant proteins research abstracts see: proteins: plant proteins research

PUBMED ID PMID:

MEDLINE DATE:

Magnesium ion-induced changes in the binding mode of adenylates to chloroplast coupling factor 1. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 80

Page Numbers: 1177-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1976

Magnesium ion-induced changes in the binding mode of adenylates to chloroplast coupling factor 1. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Magnesium ion-induced changes in the binding mode of adenylates to chloroplast coupling factor 1. Keywords Mesh Terms:

KEYWORDS: Plant Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Magnesium ion-induced changes in the binding mode of adenylates to chloroplast coupling factor 1. Information

Substance Name: Magnesium

Registry Number: 7439-95-4

Grant and Affiliation Information for Magnesium ion-induced changes in the binding mode of adenylates to chloroplast coupling factor 1.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Magnesium ion-induced changes in the binding mode of adenylates to chloroplast coupling factor 1 Related Publications

• A mechanism of respiratory control: studies with proteoliposomes containing cytochrome oxidase and bacteriorhodopsin.
• A membrane-bound ATPase from Halobacterium halobium: purification and characterization.
• Archae-opsin expressed in Escherichia coli and its conversion to purple pigment in vitro.
• Archaebacterial ATPases: relationship to other ion-translocating ATPase families examined in terms of immunological cross-reactivity.
• Dual roles of DMPC and CHAPS in the refolding of bacterial opsins in vitro.
• Effect of digitonin on photophosphorylation and light-induced H+ uptake in isolated spinach chloroplasts.
• Isolation and characterization of halorhodopsin from Halobacterium halobium.
• Magnesium ion-induced changes in the binding mode of adenylates to chloroplast coupling factor 1.
• The effect of carboxyl group modification on the chromophore regeneration of archaeopsin-1 and bacterioopsin.