Magnesium binding sites of the active conformation pf Hammerhead ribozyme.

Magnesium binding sites of the active conformation pf Hammerhead ribozyme. Research Abstract Details 

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Magnesium binding sites of the active conformation pf Hammerhead ribozyme. Abstract Text:

Mayumi Amano,

The modeling of the active conformation was done by using modeling program InsightII and Discover from the previous NMR, chemical modification and mutation data. This model structure has the reverse Watson-Crick GC base pair between C17 at the cleavage site and G5 at the core region. Two magnesium ions bind to this ribozyme. One binds to the pro-Rp oxygen of the P9 phosphate and the N7 nitrogen of G10.1, similarly to the crystal structure, the other to N3 of C3, the 2'O of C17 and the Sp-O of the scissile phosphate, while the Rp-O of that binds to NH2 of C3.

Magnesium binding sites of the active conformation pf Hammerhead ribozyme. Publishing Authors By Initials

M Amano,

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Magnesium binding sites of the active conformation pf Hammerhead ribozyme. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nucleic acids symposium series (2004)

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Page Numbers: 385-6

Journal Abbreviation: Nucleic Acids Symp Ser (Oxf)

ISSN: 1746-8272

DAY: 21

MONTH: 11

YEAR: 2007

Magnesium binding sites of the active conformation pf Hammerhead ribozyme. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Magnesium binding sites of the active conformation pf Hammerhead ribozyme.

AFFILIATION: Institute for Protein Research, Suita, Osaka 565-0781, Japan.

Country: England

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MEDLINETA: Nucleic Acids Symp Ser (Oxf)

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