MafA stability in pancreatic beta cells is regulated by glucose and is dependent on its constitutive phosphorylation at multiple sites by glycogen synthase kinase 3.

MafA stability in pancreatic beta cells is regulated by glucose and is dependent on its constitutive phosphorylation at multiple sites by glycogen synthase kinase 3. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

MafA stability in pancreatic beta cells is regulated by glucose and is dependent on its constitutive phosphorylation at multiple sites by glycogen synthase kinase 3. Abstract Text:

Song-Iee Han,Shinsaku Aramata,Kunio Yasuda,Kohsuke Kataoka,Song-Iee Han,Shinsaku Aramata,Kunio Yasuda,Kohsuke Kataoka,

Regulation of insulin gene expression by glucose in pancreatic beta cells is largely dependent on a cis-regulatory element, termed RIPE3b/C1, in the insulin gene promoter. MafA, a member of the Maf family of basic leucine zipper (bZip) proteins, is a beta-cell-specific transcriptional activator that binds to the C1 element. Based on increased C1-binding activity, MafA protein levels appear to be up-regulated in response to glucose, but the underlying molecular mechanism for this is not well understood. In this study, we show evidence supporting that the amino-terminal region of MafA is phosphorylated at multiple sites by glycogen synthase kinase 3 (GSK3) in beta cells. Mutational analysis of MafA and pharmacological inhibition of GSK3 in MIN6 beta cells strongly suggest that the rate of MafA protein degradation is regulated by glucose, that MafA is constitutively phosphorylated by GSK3, and that phosphorylation is a prerequisite for rapid degradation of MafA under low-glucose conditions. Our data suggest a new glucose-sensing signaling pathway in islet beta cells that regulates insulin gene expression through the regulation of MafA protein stability.

MafA stability in pancreatic beta cells is regulated by glucose and is dependent on its constitutive phosphorylation at multiple sites by glycogen synthase kinase 3. Publishing Authors By Initials

SI Han,S Aramata,K Yasuda,K Kataoka,SI Han,S Aramata,K Yasuda,K Kataoka,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

MafA stability in pancreatic beta cells is regulated by glucose and is dependent on its constitutive phosphorylation at multiple sites by glycogen synthase kinase 3. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular and cellular biology

VOLUME: 27

Page Numbers: 6593-605

Journal Abbreviation: Mol. Cell. Biol.

ISSN: 0270-7306

DAY: 6

MONTH: 08

YEAR: 2007

MafA stability in pancreatic beta cells is regulated by glucose and is dependent on its constitutive phosphorylation at multiple sites by glycogen synthase kinase 3. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8109087

MafA stability in pancreatic beta cells is regulated by glucose and is dependent on its constitutive phosphorylation at multiple sites by glycogen synthase kinase 3. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: MafA stability in pancreatic beta cells is regulated by glucose and is dependent on its constitutive phosphorylation at multiple sites by glycogen synthase kinase 3. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for MafA stability in pancreatic beta cells is regulated by glucose and is dependent on its constitutive phosphorylation at multiple sites by glycogen synthase kinase 3.

AFFILIATION: Laboratory of Molecular and Developmental Biology, Graduate School of Biological Science, Nara Institute of Science and Technology, Ikoma, Japan.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Cell Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

MafA stability in pancreatic beta cells is regulated by glucose and is dependent on its constitutive phosphorylation at multiple sites by glycogen synthase kinase 3 Related Publications

• A histochemical study on glycoconjugates in epithelial cells in the distal colonic mucosa of adult and developing mice.
• AUREOCHROME, a photoreceptor required for photomorphogenesis in stramenopiles.
• Adverse Effect of Cyclosporin A on Barrier Functions of Cerebral Microvascular Endothelial Cells After Hypoxia-reoxygenation Damage In Vitro.
• Bone regeneration by regulated in vivo gene transfer using biocompatible polyplex nanomicelles.
• Carbocyclization Reaction of omega-Iodo- and 1,omega-Diiodo-1-alkynes without the Loss of Iodine Atoms through a Carbenoid-Chain Process.
• Complement activation plays a key role in antibody-induced infusion toxicity in monkeys and rats.
• Crucial role of phospholipase C epsilon in skin inflammation induced by tumor-promoting phorbol ester.
• Cytotoxicity of Fas ligand against lymphoma cells with radiation-induced Fas antigen.
• Defective vascular morphogenesis and mid-gestation embryonic death in mice lacking RA-GEF-1.
• Differential Regulation of Ecdysteroidogenic P450 Gene Expression in the Silkworm, Bombyx mori.
• Effect of organic anions on the photoreaction of photoactive yellow protein.
• Gene delivery with biocompatible cationic polymer: Pharmacogenomic analysis on cell bioactivity.
• Lactosylated poly(ethylene glycol)-siRNA conjugate through acid-labile beta-thiopropionate linkage to construct pH-sensitive polyion complex micelles achieving enhanced gene silencing in hepatoma cells.
• MafA stability in pancreatic beta cells is regulated by glucose and is dependent on its constitutive phosphorylation at multiple sites by glycogen synthase kinase 3.
• Main-duct intraductal papillary mucinous adenoma of the pancreas with a large mural nodule.
• Multiple mechanisms and functions of maf transcription factors in the regulation of tissue-specific genes.
• PEGylated polyplex micelles from triblock catiomers with spatially ordered layering of condensed pDNA and buffering units for enhanced intracellular gene delivery.
• Phospholipase cepsilon Guanine nucleotide exchange factor activity and activation of rap1.
• Ras and Rap1 Activation of PLCvarepsilon Lipase Activity.
• Roles and Regulation of Transcription Factor MafA in Islet beta-cells.
• Roles of Kunitz domains in the anti-invasive effect of hepatocyte growth factor activator inhibitor type 1 in human glioblastoma cells.
• Roles of insulin-like growth factor binding protein-2 (IGFBP-2) in glioblastoma.
• Selective serotonin reuptake inhibitors fluvoxamine and paroxetine restore forced exercise-induced temperature dysregulation in ovariectomized mice.
• Structure and function of type I copper in multicopper oxidases.
• Supramolecular nanocarrier of siRNA from PEG-based block catiomer carrying diamine side chain with distinctive pKa directed to enhance intracellular gene silencing.
• Surgical repair for entire inferior vena cava occlusion in budd-Chiari syndrome.
• Unusual segmental stricture of the lower common bile duct mimicking bile duct cancer.
• [Pharmaceutical support at cardiovascular and cardiovascular surgery ward]
• [Type 1 allergy to formaldehyde in root canal sealant after dental treatment: two case reports and review of the literature]
• gamma-Tubulin-like molecules in the mouse duodenal epithelium.