Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo.

Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo. Research Abstract Details 

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Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo. Abstract Text:

James G Tidball,Michelle Wehling-Henricks,

Muscle injury or modified muscle use can stimulate muscle invasion by leucocytes that have the potential to increase tissue damage or promote tissue growth and repair. In the present investigation, we examined the role of macrophages in muscle injury, repair and regeneration during modified muscle loading. Weight-bearing was removed from the hindlimbs of mice for 10 days followed by reloading through normal ambulation. During the unloading period, soleus muscle fibre cross-section decreased by 38%. Prior to the onset of reloading, mice received a series of intraperitoneal injections of anti-F4/80, which binds a mouse macrophage surface antigen. Although anti-F4/80 injections did not affect macrophage numbers in soleus muscles at 2 days of reloading, macrophages were reduced by 86% at 4 days of reloading. Muscle membrane lysis during the reloading period did not differ at 2 days of reloading between anti-F4/80-treated mice and mice that received isotype control antibody. However, control animals showed large decreases in the number of fibres with membrane lesions at 4 days of reloading, but this membrane repair did not occur in macrophage-depleted mice. Macrophage-depletion also reduced muscle regeneration (indicated by central nucleation) and satellite cell differentiation (indicated by reductions in MyoD-expressing satellite cells) and prevented growth of muscle fibres that normally occurred in control animals between days 2 and 4 of reloading. These findings collectively show that macrophages play a significant role in muscle fibre membrane repair, regeneration and growth during increased muscle use after a period of atrophy.

Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo. Publishing Authors By Initials

JG Tidball,M Wehling-Henricks,

For similar cells: myoblasts: myoblasts, skeletal: satellite cells, skeletal muscle research abstracts see: cells: myoblasts: myoblasts, skeletal: satellite cells, skeletal muscle research

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Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of physiology

VOLUME: 578

Page Numbers: 327-36

Journal Abbreviation: J. Physiol. (Lond.)

ISSN: 0022-3751

DAY: 12

MONTH: 10

YEAR: 2006

Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo. Information

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LANGUAGE: eng

NlmUniqueID: 266262

Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo. Keywords Mesh Terms:

KEYWORDS: Satellite Cells, Skeletal Muscle

MESH TERMS: physiology

Chemical & Substance for Abstract: Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo. Information

Substance Name: MyoD Protein

Registry Number: 0

Grant and Affiliation Information for Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo.

AFFILIATION: Department of Physiological Science, 5833 Life Science Building, University of California, Los Angeles, CA 90095, USA. jtidball@physci.ucla.edu

Country: England

AGENCY: United States NIAMS

GRANT: AR47855

ACRONYM: AR

MEDLINETA: J Physiol

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