Macrophages block insulin action in adipocytes by altering expression of signaling and glucose transport proteins.

Macrophages block insulin action in adipocytes by altering expression of signaling and glucose transport proteins. Research Abstract Details 

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Macrophages block insulin action in adipocytes by altering expression of signaling and glucose transport proteins. Abstract Text:

Carey N Lumeng,Stephanie M Deyoung,Alan R Saltiel,

Obesity leads to a proinflammatory state with immune responses that include infiltration of adipose tissue with macrophages. These macrophages are believed to alter insulin sensitivity in adipocytes, but the mechanisms that underlie this effect have not been characterized. We have explored the interaction between macrophages and adipocytes in the context of both indirect and direct coculture. Macrophage-secreted factors blocked insulin action in adipocytes via downregulation of GLUT4 and IRS-1, leading to a decrease in Akt phosphorylation and impaired insulin-stimulated GLUT4 translocation to the plasma membrane. GLUT1 was upregulated with a concomitant increase in basal glucose uptake. These changes recapitulate those seen in adipose tissue from insulin-resistant humans and animal models. TNF-alpha-neutralizing antibodies partially reversed the insulin resistance produced by macrophage-conditioned media. Peritoneal macrophages and macrophage-enriched stromal vascular cells from adipose tissue also attenuated responsiveness to insulin in a manner correlating with inflammatory cytokine secretion. Adipose tissue macrophages from obese mice have an F4/80(+)CD11b(+)CD68(+)CD14(-) phenotype and form long cellular extensions in culture. Peritoneal macrophages take on similar characteristics in direct coculture with adipocytes and induce proinflammatory cytokines, suggesting that macrophage activation state is influenced by contact with adipocytes. Thus both indirect/secreted and direct/cell contact-mediated factors derived from macrophages influence insulin sensitivity in adipocytes.

Macrophages block insulin action in adipocytes by altering expression of signaling and glucose transport proteins. Publishing Authors By Initials

CN Lumeng,SM Deyoung,AR Saltiel,

For similar peptides: intercellular signaling peptides and proteins: cytokines: monokines: tumor necrosis factor-alpha research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: monokines: tumor necrosis factor-alpha research

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Macrophages block insulin action in adipocytes by altering expression of signaling and glucose transport proteins. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Endocrinology and

VOLUME: 292

Page Numbers: E166-74

Journal Abbreviation: Am. J. Physiol. Endocrinol. Me

ISSN: 0193-1849

DAY: 22

MONTH: 08

YEAR: 2006

Macrophages block insulin action in adipocytes by altering expression of signaling and glucose transport proteins. Information

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LANGUAGE: eng

NlmUniqueID: 100901226

Macrophages block insulin action in adipocytes by altering expression of signaling and glucose transport proteins. Keywords Mesh Terms:

KEYWORDS: Tumor Necrosis Factor-alpha

MESH TERMS: immunology

Chemical & Substance for Abstract: Macrophages block insulin action in adipocytes by altering expression of signaling and glucose transport proteins. Information

Substance Name: Insulin

Registry Number: 11061-68-0

Grant and Affiliation Information for Macrophages block insulin action in adipocytes by altering expression of signaling and glucose transport proteins.

AFFILIATION: Life Sciences Institute, 210 Washtenaw Ave., Ann Arbor, MI 48109, USA.

Country: United States

AGENCY: United States NICHD

GRANT: T32-HD-007513-07

ACRONYM: HD

MEDLINETA: Am J Physiol Endocrinol Metab

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