Macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor.

Macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor. Abstract Text:

Human macrophage differentiation inducing factor (DIF) can induce differentiation of human myeloid leukemic cells into macrophage-like cells in vitro. A procedure is described for purification of DIF from serum-free human monocytic leukemia THP-1 cell-conditioned medium. The procedure included concentration of a conditioned medium by ultrafiltration, lentil lectin-Sepharose affinity chromatography, and fast protein liquid chromatography using Mono S and Mono Q. DIF-A of pI 9.0 and DIF-B of pI 8.8 were obtained after a final purification with a Mono Q column, and both DIF gave a single peak with a molecular weight of approximately 51,000 determined by gel chromatography. NH2-terminal amino acid analysis of DIF-A showed a noticeable homology with murine leukemia inhibitory factor. Human DIF-A was found to induce maturation of human and murine leukemic cells into both macrophage-like cells with nitro blue tetrazolium reducing activity and phagocytic cells, but was found to suppress proliferation of these leukemic cells.

Macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 264

Page Numbers: 8941-5

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 25

MONTH: May

YEAR: 1989

Macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor. Keywords Mesh Terms:

KEYWORDS: Monocytes

MESH TERMS: physiology

Chemical & Substance for Abstract: Macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor. Information

Substance Name: Ferritins

Registry Number: 9007-73-2

Grant and Affiliation Information for Macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor.

AFFILIATION: Medical Science Laboratory, Asahi Chemical Industry Co., Ltd., Shizuoka-ken, Japan.

Country: UNITED STATES

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor Related Publications

• "Differentiation induction" culture of human leukemic myeloid cells stimulates high production of macrophage differentiation inducing factor.
• Cost-effectiveness of adjuvant chemotherapy with uracil-tegafur for curatively resected stage III rectal cancer.
• Epigenetic disregulation induces cell growth retardation in primary cultured glial cells.
• Macrophage differentiation inducing factor from human monocytic cells is equivalent to murine leukemia inhibitory factor.
• Molecular properties of the putative autolysin Atl(WM) encoded by Staphylococcus warneri M: mutational and biochemical analyses of the amidase and glucosaminidase domains.
• Mutational and biochemical analyses of the endolysin Lys(gaY) encoded by the Lactobacillus gasseri JCM 1131(T) phage phigaY.
• Promoter/repressor system of Lactobacillus plantarum phage og1e: characterization of the promoters pR49-pR-pL and overproduction of the cro-like protein cng in Escherichia coli.
• Unresectable adenoid cystic carcinoma of the trachea treated with chemoradiotherapy.
• [Recent progress on radiotherapy for head and neck neoplasms]