Lysozyme refolding with cyclodextrins: structure-activity relationship.

Lysozyme refolding with cyclodextrins: structure-activity relationship. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Lysozyme refolding with cyclodextrins: structure-activity relationship. Abstract Text:

A Desai,C Lee,L Sharma,A Sharma,

Cyclodextrins (CDs), in the presence or absence of detergents, have been reported to suppress aggregate formation during the refolding of a number of proteins. A structure-activity relationship study between CD chemistry and refolding of lysozyme was performed and compared to carbonic anhydrase, in order to better understand the mechanism of CD-assisted protein refolding and to identify CDs that could function as good protein folding agents. Among the natural CDs, which have only hydroxyl groups, alpha-CD, with a smaller cavity size was more effective than the oligosaccharide with a larger cavity, gamma-CD. Replacement of the hydroxyls with other functional groups did not improve, but could seriously interfere, with the lysozyme refolding ability of alpha-CD. In case of gamma-CD, substitution of its hydroxyls with other groups either enhanced or diminished its refolding capability towards lysozyme. In general, neutral CDs were better refolding agents than the charged sugars. The presence of anionic substituents like carboxyl and phosphate groups actually promoted aggregate formation and completely abolished the sugar's refolding ability. This effect was more pronounced with lysozyme than with carbonic anhydrase. CDs with cationic functional groups did not show any significant effects on lysozyme refolding. The presence of both anionic and cationic substituents on the same CD molecule was found to partially restore its renaturation ability. Electrophoresis data indicate that CDs, which promoted lysozyme refolding, arrested aggregation at the stage of smaller soluble aggregates. Interestingly, the structure-activity relationship observed with lysozyme was quite similar to that reported for a non-disulfide protein, carbonic anhydrase. These results suggest that the effects of CDs on protein refolding are attributed to their ability to suppress aggregation of proteins. CDs may show properties similar to chaotropic agents, which may help explain their anti-aggregation and protein refolding ability. Besides alpha-CD, a number of other neutral CDs were found to be effective protein folding aids.

Lysozyme refolding with cyclodextrins: structure-activity relationship. Publishing Authors By Initials

A Desai,C Lee,L Sharma,A Sharma,

For similar polycyclic compounds: macrocyclic compounds: cyclodextrins: beta-cyclodextrins research abstracts see: polycyclic compounds: macrocyclic compounds: cyclodextrins: beta-cyclodextrins research

PUBMED ID PMID:

MEDLINE DATE:

Lysozyme refolding with cyclodextrins: structure-activity relationship. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochimie

VOLUME: 88

Page Numbers: 1435-45

Journal Abbreviation: Biochimie

ISSN: 0300-9084

DAY: 24

MONTH: 05

YEAR: 2006

Lysozyme refolding with cyclodextrins: structure-activity relationship. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 1264604

Lysozyme refolding with cyclodextrins: structure-activity relationship. Keywords Mesh Terms:

KEYWORDS: beta-Cyclodextrins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Lysozyme refolding with cyclodextrins: structure-activity relationship. Information

Substance Name: Carbonic Anhydrases

Registry Number: EC 4.2.1.1

Grant and Affiliation Information for Lysozyme refolding with cyclodextrins: structure-activity relationship.

AFFILIATION: Department of Chemistry, Central Michigan University, Dow 346, Mt. Pleasant, MI 48859, USA.

Country: France

AGENCY: United States NIGMS

GRANT: R15GM065975-01

ACRONYM: GM

MEDLINETA: Biochimie

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Lysozyme refolding with cyclodextrins: structure-activity relationship Related Publications

• Behavioral manifestations of sturge-weber syndrome: a case report.
• Body size and pelvic area relationships at puberty and two-month-pregnancy in buffalo - heifers.
• Calcium-modulated rod outer segment membrane guanylate cyclase type 1 transduction machinery in the testes.
• Ceramide synthase inhibition by fumonisin B1 treatment activates sphingolipid-metabolizing systems in mouse liver.
• Cerebellopointine angle medulloblastoma.
• Conserved F84 and P86 residues in alphaB-crystallin are essential to effectively prevent the aggregation of substrate proteins.
• Current status of rotational atherectomy.
• Decreased expressions of c-myc and H-ras oncogenes in vitamin E succinate induced morphologically differentiated murine B-16 melanoma cells in culture.
• Dens evaginatus of anterior teeth (talon cusp) associated with other odontogenic anomalies.
• Dystocia due to urinary bladder carcinoma in two water buffaloes (bubalus bubalis) - Clinical case report.
• Effect of vitamin E succinate and a cAMP-stimulating agent on the expression of c-myc and N-myc and H-ras in murine neuroblastoma cells.
• Exploring evolving concepts and challenges in forensic psychiatry.
• Identification of a hypothetical membrane protein interactor of ribosomal phosphoprotein P0.
• In vivo pelvimetry in buffaloes (bos bubalus ).
• Induction of estrus in anestrous cows treated with 'Fertivet'.
• Influence of body mass index and self-administration of hCG on the outcome of IVF cycles: a prospective cohort study.
• Insulin resistance and clinical aspects of non-alcoholic steatohepatitis (NASH).
• Interleukins and STAT signaling.
• Lysozyme refolding with cyclodextrins: structure-activity relationship.
• Metalloantimalarials: synthesis and characterization of a novel agent possessing activity against Plasmodium falciparum.
• Novel frequenin-modulated Ca2+-signaling membrane guanylate cyclase (ROS-GC) transduction pathway in bovine hippocampus.
• Pituitary abscess associated with prolactinoma.
• Right hemothorax from leaking aortocoronary vein graft giant aneurysm.
• S-adenosylmethionine or 5'-methylthioadenosine are unable to prevent fumonisin B1 hepatotoxicity in mice despite increased oxidation in liver.
• Single photon emission computed tomography and positron emission tomography imaging of multi-drug resistant P-glycoprotein--monitoring a transport activity important in cancer, blood-brain barrier function and Alzheimer's disease.
• Studies on follicular patterns in buffalo-heifers.
• Studies on lactic dehydrogenase and sorbitol dehydrogenase release in relation to deep freezing of buffalo semen in certain extenders.
• Studies on serum progesterone levels in relation to occurrence of uterine torsion in buffaloes (Bubalus bubalis ).
• Thyroglossal duct remnant: seven years analysis.
• Treatment resistance in unipolar depression: Is it an iatrogenic phenomenon caused by antidepressant treatment of patients with a bipolar diathesis?