Lysozyme loading and release from hydrogels carrying pendant phosphate groups.

Lysozyme loading and release from hydrogels carrying pendant phosphate groups. Research Abstract Details 

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Lysozyme loading and release from hydrogels carrying pendant phosphate groups. Abstract Text:

K Nakamae,T Nizuka,T Miyata,M Furukawa,T Nishino,K Kato,T Inoue,A S Hoffman,Y Kanzaki,

To develop a polymeric matrix for efficiently loading cationic biomolecules, polyelectrolyte hydrogels carrying pendant phosphate groups were synthesized by copolymerizing 2-methacryloyloxyethyl dihydrogen phosphate with N-isopropylacrylamide and N, N1-methylene-bis-acrylamide. The phosphate-carrying monomer yielded anionic hydrogels, which formed ionic complexes with the cationic protein, lysozyme. It was shown that the amount of complexed lysozyme reached 2.1 g g-1 dry gel, corresponding to 1.3 x 10(-3) mol phosphate group per gram lysozyme, when 40 mol% of phosphate-carrying monomer was incorporated in a hydrogel. When the hydrogel complexed with lysozyme was placed in deionized water and various KCl solutions, of varying concentrations of up to 0.5 M KCl, no lysozyme was released in deionized water, while increasing amounts of lysozyme were released as the KCl concentration increased. This confirmed that lysozyme was loaded in the hydrogel through electrostatic interactions. It was further found that the complexed lysozyme retained its enzymatic activity after being released from the hydrogel. These results suggest the use of this system for the controlled release of cationic protein drugs.

Lysozyme loading and release from hydrogels carrying pendant phosphate groups. Publishing Authors By Initials

K Nakamae,T Nizuka,T Miyata,M Furukawa,T Nishino,K Kato,T Inoue,AS Hoffman,Y Kanzaki,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research

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Lysozyme loading and release from hydrogels carrying pendant phosphate groups. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 9

Page Numbers: 43-53

Journal Abbreviation:

ISSN: 0920-5063

DAY: 20

MONTH: 02

YEAR: 1997

Lysozyme loading and release from hydrogels carrying pendant phosphate groups. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9007393

Lysozyme loading and release from hydrogels carrying pendant phosphate groups. Keywords Mesh Terms:

KEYWORDS: Protein Binding

MESH TERMS: chemical synthesis

Chemical & Substance for Abstract: Lysozyme loading and release from hydrogels carrying pendant phosphate groups. Information

Substance Name: Muramidase

Registry Number: EC 3.2.1.17

Grant and Affiliation Information for Lysozyme loading and release from hydrogels carrying pendant phosphate groups.

AFFILIATION: Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, Japan.

Country: NETHERLANDS

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MEDLINETA: J Biomater Sci Polym Ed

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