Lymphocytes from enlarged iliac lymph nodes as fusion partners for the production of monoclonal antibodies after a single tail base immunization attempt.

Lymphocytes from enlarged iliac lymph nodes as fusion partners for the production of monoclonal antibodies after a single tail base immunization attempt. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Lymphocytes from enlarged iliac lymph nodes as fusion partners for the production of monoclonal antibodies after a single tail base immunization attempt. Abstract Text:

Yoshikazu Sado,Satoko Inoue,Yasuko Tomono,Hiroyuki Omori,

A novel method of preparing hybridomas producing mouse monoclonal antibodies was -established, called "the mouse iliac lymph node method". Lymphocytes from enlarged iliac lymph nodes from mice injected intramuscularly at the tail base with an emulsion of antigen and Freund's adjuvant were used for cell fusion. For the most part, lymph node lymphocytes from two mice were used for a single cell fusion attempt. Ovalbumin was used as the antigen and the results of fusion were compared with the results of a previous report (Cell Struct. Funct. 20; 151-156, 1995). Approximately 100 positive wells producing antibody of interest were identified using this method. By comparison, approximately 10 positive wells were identified using the more conventional mouse spleen method after three immunization injections. The relative proportions of hybridomas producing IgM, IgG1, IgG2a, IgG2b, and IgG3, following fusion using iliac lymph node lymphocytes obtained 14 days after injection were 14.0%, 78.7%, 3.2%, 3.5% and 0.5%, respectively. This method demonstrated the following advantages: (1) a single injection of the antigen emulsion was sufficient, (2) the lymph nodes were ready for use 14 days after injection, and (3) a high yield of positive hybridomas was obtained.

Lymphocytes from enlarged iliac lymph nodes as fusion partners for the production of monoclonal antibodies after a single tail base immunization attempt. Publishing Authors By Initials

Y Sado,S Inoue,Y Tomono,H Omori,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Lymphocytes from enlarged iliac lymph nodes as fusion partners for the production of monoclonal antibodies after a single tail base immunization attempt. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta histochemica et cytochemica

VOLUME: 39

Page Numbers: 89-94

Journal Abbreviation:

ISSN: 0044-5991

DAY: 26

MONTH: 04

YEAR: 2006

Lymphocytes from enlarged iliac lymph nodes as fusion partners for the production of monoclonal antibodies after a single tail base immunization attempt. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 147110

Lymphocytes from enlarged iliac lymph nodes as fusion partners for the production of monoclonal antibodies after a single tail base immunization attempt. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Lymphocytes from enlarged iliac lymph nodes as fusion partners for the production of monoclonal antibodies after a single tail base immunization attempt. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Lymphocytes from enlarged iliac lymph nodes as fusion partners for the production of monoclonal antibodies after a single tail base immunization attempt.

AFFILIATION: Shigei Medical Research Institute, 2117 Yamada, Okayama 701-0202, Japan.

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Acta Histochem Cytochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Lymphocytes from enlarged iliac lymph nodes as fusion partners for the production of monoclonal antibodies after a single tail base immunization attempt Related Publications

• Analysis of 95 cases of squamous cell carcinoma of the external and middle ear.
• Development and evaluation of intermediate frequency magnetic field exposure system for studies of in vitro biological effects.
• Different effect of aflatoxin on the induction of tryptophan oxygenase and of microsomal drug-hydroxylase system.
• Effect of fibroblasts on epithelial regeneration on the surface of a bioengineered trachea.
• Effects of thyroid hormone on the hydroxylation of progesterone in liver microsomes of male and female rats.
• Genomic organization of the human phosphodiesterase PDE11A gene. Evolutionary relatedness with other PDEs containing GAF domains.
• Identification of rat cyclic nucleotide phosphodiesterase 11A (PDE11A): comparison of rat and human PDE11A splicing variants.
• Identification of the specific labile sites in the 180 kDa catalytic polypeptide of the Drosophila melanogaster DNA polymerase alpha-primase complex.
• Isolation and characterization of two novel phosphodiesterase PDE11A variants showing unique structure and tissue-specific expression.
• Lymphocytes from enlarged iliac lymph nodes as fusion partners for the production of monoclonal antibodies after a single tail base immunization attempt.
• Regeneration of the trachea using a bioengineered scaffold with adipose-derived stem cells.
• Regeneration of tracheal epithelium utilizing a novel bipotential collagen scaffold.
• Sentinel node mapping for node positive oral cancer: potential to predict multiple metastasis.
• Statistical issues in the use of the comet assay.
• [Correlation between interactions of younger elderly women with close non-family friends and neighbors and health-related QOL]
• [Improving problems in patient education by means of TA]
• [Marrow-tympanum connections in fetuses and infants]
• [On the neoplastic growth of lungs of mice inoculated with virus HST.]
• [The nurses' station as the basis and liaison point in nursing]