Lymphocyte-increasing action and hypocalcemic action of parotid gland extract.

Lymphocyte-increasing action and hypocalcemic action of parotid gland extract. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Lymphocyte-increasing action and hypocalcemic action of parotid gland extract. Abstract Text:

I Suzuki,H Inaba,Y Naito,T Mizutani,A Mizutani,

It has previously been shown in our laboratory that the hypocalcemic substances purified from the thymus have a potent lymphocyte-increasing action in mice. Then, lymphocyte-increasing activity was examined with bovine parotid gland extracts, which showed a hypocalcemic activity as potent as that of the thymus extracts. The lymphocyte-increasing activity was assayed in the littermates of neonatal mice of Swiss-Webster strain; the materials used for this experiment were purified preparations and several fractions obtained from the parotid gland extracts in the course of purification. It was found that the potency of lymphocyte-increasing activity rose approximately in parallel with the rise in hypocalcemic activity with progress in purification. The final product, which was purified from the gland by isoelectric precipitation at pH 5.4, fractional precipitation with ammonium sulfate, chromatography on DEAE-cellulose, gel filtration on Sepharose 6B and preparative disc electrophoresis, gave a single band in polyacrylamide gel electrophoresis. Its intravenous injection in rabbits, in a dose of 10 mug/kg, produced a significant lowering in serum calcium (percent decrease 10.24 +/- 1.06%) compared with control animals given an injection of physiological saline. Intraperitoneal injection of this purified product, in a dose of 0.5 mug/mouse, in the littermates of neonatal mice, also produced a significant increase (ratio of lymphocytes to polymorphs 2.47 +/- 0.07) in lymphocytes compared with control animals injected with physiological saline (L/P ratio 1.57 +/- 0.05). These facts suggest that this purified protein fraction inherently contains both activities, but the possibility cannot be ruled out of slight contamination by a substance having a high activity. On the other hand, a fraction having no activity for lowering serum calcium but which had activity for increasing the lymphocytes was obtained. This is the first paper to report the presence of lymphocyte-increasing substances in the bovine parotid gland and the purification of one of the substances from the gland.

Lymphocyte-increasing action and hypocalcemic action of parotid gland extract. Publishing Authors By Initials

I Suzuki,H Inaba,Y Naito,T Mizutani,A Mizutani,

For similar complex mixtures: tissue extracts research abstracts see: complex mixtures: tissue extracts research

PUBMED ID PMID:

MEDLINE DATE:

Lymphocyte-increasing action and hypocalcemic action of parotid gland extract. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 78

Page Numbers: 697-703

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1975

Lymphocyte-increasing action and hypocalcemic action of parotid gland extract. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Lymphocyte-increasing action and hypocalcemic action of parotid gland extract. Keywords Mesh Terms:

KEYWORDS: Tissue Extracts

MESH TERMS: physiology

Chemical & Substance for Abstract: Lymphocyte-increasing action and hypocalcemic action of parotid gland extract. Information

Substance Name: Tissue Extracts

Registry Number: 0

Grant and Affiliation Information for Lymphocyte-increasing action and hypocalcemic action of parotid gland extract.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Lymphocyte-increasing action and hypocalcemic action of parotid gland extract Related Publications

• A case of Adamantiades-Behçet disease with ischemic optic neuritis (posterior optic neuropathy).
• Adsorption chromatography of nucleic acids on silicone-coated porous glass.
• Chemical modification of pseudouridylic acid in isoeleucine transfer ribonucleic acid from Torulopsis utilis with a radioactive carbodiimide.
• Chemical modification of pseudouridylic acid in valine transfer ribonucleic acid I from Torulopsis utilis with a radioactive carbodiimide.
• Effect of low-intensity back exercise on quality of life and back extensor strength in patients with osteoporosis: a randomized controlled trial.
• Isolation of Novel Adenovirus from Fruit Bat (Pteropus dasymallus yayeyamae).
• Lymphocyte-increasing action and hypocalcemic action of parotid gland extract.
• Magnetic resonance (MR) imaging-induced deep second-degree burns of lower extremities by conducting loop.
• Necrobiotic xanthogranuloma with paraproteinemia; an atypical case.
• Neuroendocrine carcinoma of the stomach with peripancreatic lymph node metastases successfully treated with pancreaticoduodenectomy.
• Oxidative stress in neurodegeneration in dentatorubral-pallidoluysian atrophy.
• Primary protein response after ligand photodissociation in carbonmonoxy myoglobin.
• Rapid determination of viral RNA sequences in mosquitoes collected in the field.
• Serum granulysin is elevated in patients with hemophagocytic lymphohistiocytosis.
• Silver stainings distinguish Lewy bodies and glial cytoplasmic inclusions: comparison between Gallyas-Braak and Campbell-Switzer methods.
• Soluble TWEAK is markedly elevated in hemophagocytic lymphohistiocytosis.
• Structure determination of structurally complex Ag36Li64 gamma-brass.
• Target scattering waves emitted from a time reversal array in shallow water.
• The reversible chemical modification of valine transfer ribonucleic acid I from Torulopsis with a radioactive carbodiimide.
• [Contact lens wearing and corneal changes]
• [Correlation between molecular evolution of P450s and evolution of plant secondary metabolism]
• [Etiology on the formation of the dimples of the central epithelium of the cornea associated with the wearing of corneal contact lens]
• [Leukoencephalopathy]
• [Man and language]
• [Mechanism of DNA damage and apoptosis induced by anticancer drugs through generation of reactive oxygen species]
• [OPTICAL ZONE OF CONTACT LENSES.]
• [Remarks on the examination of nursing needs; with special reference to the instruction of pre- and postoperative nursing]
• [The clinical effect of antibiotics in the macrolide family on bronchial asthma. Non-antimicrobial actions of oleandomycin [author's transl)]
• [The clinical importance of contact lens hydration]
• [The effects of contact lenses on the human cornea. I. Clinical experiment and corneal changes]