Luteinizing hormone signaling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway.

Luteinizing hormone signaling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Luteinizing hormone signaling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway. Abstract Text:

Sara Panigone,Minnie Hsieh,Maoyong Fu,Luca Persani,Marco Conti,

LH activates a cascade of signaling events that are propagated throughout the ovarian preovulatory follicle to promote ovulation of a mature egg. Critical to LH-induced ovulation is the induction of epidermal growth factor (EGF)-like growth factors and transactivation of EGF receptor (EGFR) signaling. Because the timing of this transactivation has not been well characterized, we investigated the dynamics of LH regulation of the EGF network in cultured follicles. Preovulatory follicles were cultured with or without recombinant LH and/or specific inhibitors. EGFR and MAPK phosphorylation were examined by immunoprecipitation and Western blot analyses. By semiquantitative RT-PCR, increases in amphiregulin and epiregulin mRNAs were detected 30 min after recombinant LH stimulation of follicles and were maximal after 2 h. LH-induced EGFR phosphorylation also increased after 30 min and reached a maximum at 2 h. EGFR activation precedes oocyte maturation and is cAMP dependent, because forskolin similarly activated EGFR. LH-induced EGFR phosphorylation was sensitive to AG1478, an EGFR kinase inhibitor, and to inhibitors of matrix metalloproteases GM6001 and TNFalpha protease inhibitor-1 (TAPI-1), suggesting the involvement of EGF-like growth factor shedding. LH- but not amphiregulin-induced oocyte maturation and EGFR phosphorylation were sensitive to protein synthesis inhibition. When granulosa cells were cultured with a combination of neutralizing antibodies against amphiregulin, epiregulin, and betacellulin, EGFR phosphorylation and MAPK activation were inhibited. In cultured follicles, LH-induced MAPK activation was partially inhibited by AG1478 and GM6001, indicating that this pathway is regulated in part by the EGF network but also involves additional pathways. Thus, complex mechanisms are involved in the rapid amplification and propagation of the LH signal within preovulatory follicles and include the early activation of the EGF network.

Luteinizing hormone signaling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway. Publishing Authors By Initials

S Panigone,M Hsieh,M Fu,L Persani,M Conti,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Luteinizing hormone signaling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular endocrinology (Baltimore, Md.)

VOLUME: 22

Page Numbers: 924-36

Journal Abbreviation: Mol. Endocrinol.

ISSN: 0888-8809

DAY: 10

MONTH: 01

YEAR: 2008

Luteinizing hormone signaling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8801431

Luteinizing hormone signaling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Luteinizing hormone signaling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Luteinizing hormone signaling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway.

AFFILIATION: Department of Obstetrics, Gynecology and Reproductive Sciences, University of California at San Francisco, 513 Parnassus, San Francisco, California 94143-0556. ContiM@obgyn.ucsf.edu.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Endocrinol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Luteinizing hormone signaling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway Related Publications

• 2-deoxy-2-[F-18]fluoro-D-glucose-positron emission tomography/computed tomography imaging evaluation of esophageal cancer.
• Academic education in complementary medicine: a tuscan methodological perspective.
• Arrhythmias in the pregnant patient: current concepts in evaluation and management.
• Assessing calibration uncertainty in molecular dating: the assignment of fossils to alternative calibration points.
• Biodistribution and PET imaging of [(18)F]-fluoroadenosine derivatives.
• Cardiac resynchronization therapy for treatment of heart failure in the elderly.
• Efficacy of donkey's milk in treating highly problematic cow's milk allergic children: an in vivo and in vitro study.
• In vivo evaluation of 2'-deoxy-2'-[(18)F]fluoro-5-iodo-1-beta-D-arabinofuranosyluracil ([18F]FIAU) and 2'-deoxy-2'-[18F]fluoro-5-ethyl-1-beta-D-arabinofuranosyluracil ([18F]FEAU) as markers for suicide gene expression.
• Initial presenting electrocardiogram as determinant for hospital admission in patients presenting to the emergency department with chest pain: a pilot investigation.
• Interleukin-1 system in CNS stress: seizures, fever, and neurotrauma.
• Interleukin-18 controls energy homeostasis by suppressing appetite and feed efficiency.
• Investigating radial glia in vitro.
• Luteinizing hormone-dependent activation of the epidermal growth factor network is essential for ovulation.
• Milk fat globule membrane components--a proteomic approach.
• Morbid obesity, cardiac disease, and bariatric surgery.
• Not Available
• Response of spinal myoclonus to a combination therapy of autogenic training and biofeedback.
• ST-Elevation Myocardial Infarction: Thrombus Burden and Prognosis.
• Scales to climb borderline personalities: when science goes nowhere.
• Signaling from beta(1)- and beta(2)-adrenergic receptors is defined by differential interactions with PDE4.
• Structural health monitoring by extraction of coherent guided waves from diffuse fields.
• Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together.
• Testing association between disease and multiple SNPs in a candidate gene.
• [Colpocytological findings after vaginal injection of acetylcholine and histamine.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Seroprevalence of antibodies against Trypanosoma cruzi in 13 departments of Uruguay]
• [Should we PCI in patients with silent myocardial ischemia?]