LPS induces translocation of TLR4 in amniotic epithelium.

LPS induces translocation of TLR4 in amniotic epithelium. Research Abstract Details 

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LPS induces translocation of TLR4 in amniotic epithelium. Abstract Text:

K M Adams,J Lucas,R P Kapur,A M Stevens,

Toll-like receptor 4 (TLR4) mediates lipopolysaccharide (LPS) induced immune responses, which may contribute to preterm labor associated with intraamniotic gram-negative bacterial infections. The study objective was to investigate gestational age and LPS-induced changes in TLR4 subcellular localization within amniotic epithelium, the first line of host defense against intraamniotic bacteria. TLR4 localization in amniotic epithelium was assessed using immunohistochemistry on 24 placentas of different gestational ages: first trimester (n=6), second trimester (n=6), and third trimester (n=12). Immunofluorescence was used to determine TLR4 localization following ex vivo LPS stimulation of amnion from women undergoing cesarean section without labor at term. TLR4 was expressed in the cytoplasm of amniotic epithelium starting at 9weeks with apical polarization by 25weeks gestation. TLR4 localization to the basal membrane was significantly associated with chorioamnionitis (p=0.01). After LPS stimulation, TLR4 was expressed sequentially within the apical membrane, cytoplasm, and finally in the basal cellular compartment. This suggests that TLR4 expression in amniotic epithelium is poised to monitor amniotic fluid for pathogens. TLR4 translocation to the basal membrane may decrease LPS signaling early in an infection, but allow the amniotic epithelium to remain competent to invasive or intracellular bacteria.

LPS induces translocation of TLR4 in amniotic epithelium. Publishing Authors By Initials

KM Adams,J Lucas,RP Kapur,AM Stevens,

For similar proteins: membrane proteins: receptors, cell surface: receptors, immunologic: receptors, pattern recognition: toll-like receptors: toll-like receptor 4 research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, immunologic: receptors, pattern recognition: toll-like receptors: toll-like receptor 4 research

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LPS induces translocation of TLR4 in amniotic epithelium. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Placenta

VOLUME: 28

Page Numbers: 477-81

Journal Abbreviation:

ISSN: 0143-4004

DAY: 19

MONTH: 10

YEAR: 2006

LPS induces translocation of TLR4 in amniotic epithelium. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8006349

LPS induces translocation of TLR4 in amniotic epithelium. Keywords Mesh Terms:

KEYWORDS: Toll-Like Receptor 4

MESH TERMS: metabolism

Chemical & Substance for Abstract: LPS induces translocation of TLR4 in amniotic epithelium. Information

Substance Name: Toll-Like Receptor 4

Registry Number: 0

Grant and Affiliation Information for LPS induces translocation of TLR4 in amniotic epithelium.

AFFILIATION: Department of Obstetrics & Gynecology, University of Washington, Seattle, WA, USA. adamsk@u.washington.edu <adamsk@u.washington.edu>

Country: England

AGENCY: United States NICHD

GRANT: HD01264

ACRONYM: HD

MEDLINETA: Placenta

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