Loss of stem cell regenerative capacity within aged niches.

Loss of stem cell regenerative capacity within aged niches. Research Abstract Details 

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Loss of stem cell regenerative capacity within aged niches. Abstract Text:

Morgan E Carlson,Irina M Conboy,

This work uncovers novel mechanisms of aging within stem cell niches that are evolutionarily conserved between mice and humans and affect both embryonic and adult stem cells. Specifically, we have examined the effects of aged muscle and systemic niches on key molecular identifiers of regenerative potential of human embryonic stem cells (hESCs) and post-natal muscle stem cells (satellite cells). Our results reveal that aged differentiated niches dominantly inhibit the expression of Oct4 in hESCs and Myf-5 in activated satellite cells, and reduce proliferation and myogenic differentiation of both embryonic and tissue-specific adult stem cells (ASCs). Therefore, despite their general neoorganogenesis potential, the ability of hESCs, and the more differentiated myogenic ASCs to contribute to tissue repair in the old will be greatly restricted due to the conserved inhibitory influence of aged differentiated niches. Significantly, this work establishes that hESC-derived factors enhance the regenerative potential of both young and, importantly, aged muscle stem cells in vitro and in vivo; thus, suggesting that the regenerative outcome of stem cell-based replacement therapies will be determined by a balance between negative influences of aged tissues on transplanted cells and positive effects of embryonic cells on the endogenous regenerative capacity. Comprehensively, this work points toward novel venues for in situ restoration of tissue repair in the old and identifies critical determinants of successful cell-replacement therapies for aged degenerating organs.

Loss of stem cell regenerative capacity within aged niches. Publishing Authors By Initials

ME Carlson,IM Conboy,

For similar cells: stem cells research abstracts see: cells: stem cells research

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Loss of stem cell regenerative capacity within aged niches. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Aging cell

VOLUME: 6

Page Numbers: 371-82

Journal Abbreviation: Aging Cell

ISSN: 1474-9718

DAY: 23

MONTH: 03

YEAR: 2007

Loss of stem cell regenerative capacity within aged niches. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101130839

Loss of stem cell regenerative capacity within aged niches. Keywords Mesh Terms:

KEYWORDS: Stem Cells

MESH TERMS: cytology

Chemical & Substance for Abstract: Loss of stem cell regenerative capacity within aged niches. Information

Substance Name: Pou5f1 protein, mouse

Registry Number: 0

Grant and Affiliation Information for Loss of stem cell regenerative capacity within aged niches.

AFFILIATION: Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94702, USA.

Country: England

AGENCY: United States NIA

GRANT: R21 AG27892

ACRONYM: AG

MEDLINETA: Aging Cell

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