Localization of Smads, the TGF-beta family intracellular signaling components during endochondral ossification.

Localization of Smads, the TGF-beta family intracellular signaling components during endochondral ossification. Research Abstract Details 

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Localization of Smads, the TGF-beta family intracellular signaling components during endochondral ossification. Abstract Text:

T Sakou,T Onishi,T Yamamoto,T Nagamine,T k Sampath,P Ten Dijke,

Members of the transforming growth factor-beta (TGF-beta) family transduce signals from the cell membrane to the nucleus via specific type I and type II receptors and Smad proteins. Smad1 and Smad5 mediate intracellular signaling of bone morphogenetic protein (BMP), whereas Smad2 and Smad3 transduce TGF-beta signaling. Smad4 is a common mediator required for both pathways. Smad6 and Smad7 inhibit signaling by members of the TGF-beta superfamily. Here, we examined the expression of Smad1 to Smad7 proteins during endochondral ossification of epiphyseal plate of growing rats using immunohistochemical techniques. The expression of Smad proteins was correlated with the expression of TGF-beta1 and its receptors, and BMP-2/4 and BMP receptors. The results show that TGF-beta1 and BMP-2/4 were actively expressed in chondrocytes that are undergoing proliferation and maturation, which overlaps with expression of their corresponding type I and type II receptors. The Smads, however, exhibited a distinct expression pattern, respectively. For example, Smad1 and Smad5 were highly expressed in proliferating chondrocytes and in those chondrocytes that are undergoing maturation. The TGF-beta/activin-restricted Smads were also expressed in a nearly complementary fashion; Smad2 was strongly expressed in proliferating chondrocytes, whereas Smad3 was strongly observed in maturing chondrocytes. Smad4 was broadly expressed in all zones of epiphyseal plate. Inhibitory Smads, Smad6 and Smad7, were strongly expressed in the zone of cartilage that contained mature chondrocytes. Our findings show a colocalization of the pathway-restricted and inhibitory Smads with activating ligands or ligands whose action they antagonize and their receptors in various zones of epiphyseal growth plate, suggesting that TGF-beta superfamily Smad signaling pathways plays a morphogenic role during endochondral bone formation.

Localization of Smads, the TGF-beta family intracellular signaling components during endochondral ossification. Publishing Authors By Initials

T Sakou,T Onishi,T Yamamoto,T Nagamine,T Sampath,P Ten Dijke,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Localization of Smads, the TGF-beta family intracellular signaling components during endochondral ossification. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of bone and mineral research : the officia

VOLUME: 14

Page Numbers: 1145-52

Journal Abbreviation: J. Bone Miner. Res.

ISSN: 0884-0431

DAY: 19

MONTH: Jul

YEAR: 1999

Localization of Smads, the TGF-beta family intracellular signaling components during endochondral ossification. Information

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LANGUAGE: eng

NlmUniqueID: 8610640

Localization of Smads, the TGF-beta family intracellular signaling components during endochondral ossification. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: metabolism

Chemical & Substance for Abstract: Localization of Smads, the TGF-beta family intracellular signaling components during endochondral ossification. Information

Substance Name: transforming growth factor-beta type II

Registry Number: EC 2.7.11.30

Grant and Affiliation Information for Localization of Smads, the TGF-beta family intracellular signaling components during endochondral ossification.

AFFILIATION: Department of Orthopaedic Surgery, Faculty of Medicine, Kagoshima University, Kagoshima, Japan.

Country: UNITED STATES

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MEDLINETA: J Bone Miner Res

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