Localization of silver deposits on Pneumocystis carinii treated with Gomori's methenamine silver nitrate stain.

Localization of silver deposits on Pneumocystis carinii treated with Gomori's methenamine silver nitrate stain. Research Abstract Details 

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Localization of silver deposits on Pneumocystis carinii treated with Gomori's methenamine silver nitrate stain. Abstract Text:

H Yoshikawa,Y Yoshida,

The question which portion of the pellicle of Pneumocystis carinii is actually stained by the Gomori's methenamine silver nitrate (GMS) method was solved with the aid of electron microscopy. Silver particles were specifically deposited on the electron-lucent middle layer of the precyst, cyst and ruptured cyst, whereas such deposition was not found on the pellicle of the trophozoite, probably due to the absence of the electron-lucent middle layer in the trophozoite. These deposits increased in proportion to the duration of staining and to the thickness of the lucent middle layer. Therefore, the lighter colored organisms as observed under the light microscope are considered to be the precysts because they have thinner GMS-positive middle layers than those of cysts. So-called parentheses-like structures or rings are one of the most characteristic light microscopic features of P. carinii cysts when stained with GMS. The present study indicates that these structures must be of cyst wall origin rather than of cytoplasm origin, because the thickened part of the cyst wall showed a dense deposition of silver particles, probably corresponding to the parentheses-like structure. On the other hand we also observed, in the endogeny form of trophozoite, a thin GMS-positive layer in the parent pellicle though this pellicle showed no differences from those of daughter and common trophozoites when observed by means of ultrathin section microscopy.

Localization of silver deposits on Pneumocystis carinii treated with Gomori's methenamine silver nitrate stain. Publishing Authors By Initials

H Yoshikawa,Y Yoshida,

For similar investigative techniques: clinical laboratory techniques: cytological techniques: histocytological preparation techniques: staining and labeling research abstracts see: investigative techniques: clinical laboratory techniques: cytological techniques: histocytological preparation techniques: staining and labeling research

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Localization of silver deposits on Pneumocystis carinii treated with Gomori's methenamine silver nitrate stain. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zentralblatt für Bakteriologie, Mikrobiologie, und

VOLUME: 264

Page Numbers: 363-72

Journal Abbreviation:

ISSN: 0176-6724

DAY: 12

MONTH: May

YEAR: 1987

Localization of silver deposits on Pneumocystis carinii treated with Gomori's methenamine silver nitrate stain. Information

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LANGUAGE: eng

NlmUniqueID: 8403032

Localization of silver deposits on Pneumocystis carinii treated with Gomori's methenamine silver nitrate stain. Keywords Mesh Terms:

KEYWORDS: Staining and Labeling

MESH TERMS: analysis

Chemical & Substance for Abstract: Localization of silver deposits on Pneumocystis carinii treated with Gomori's methenamine silver nitrate stain. Information

Substance Name: Silver Nitrate

Registry Number: 7761-88-8

Grant and Affiliation Information for Localization of silver deposits on Pneumocystis carinii treated with Gomori's methenamine silver nitrate stain.

AFFILIATION: Dept. of Medical Zoology, Kyoto Prefectural University of Medicine, Japan.

Country: GERMANY, WEST

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MEDLINETA: Zentralbl Bakteriol Mikrobiol

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