Localization of Haemophilus ducreyi in naturally acquired chancroidal ulcers.

Localization of Haemophilus ducreyi in naturally acquired chancroidal ulcers. Research Abstract Details 

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Localization of Haemophilus ducreyi in naturally acquired chancroidal ulcers. Abstract Text:

Margaret E Bauer,Carisa A Townsend,Allan R Ronald,Stanley M Spinola,

Haemophilus ducreyi causes the sexually transmitted genital ulcer disease chancroid. In human inoculation experiments, bacteria colocalize with neutrophils and macrophages but remain extracellular. The organism also colocalizes with collagen and fibrin but not with keratinocytes, fibroblasts, laminin, or fibronectin. These relationships are established by 48 h postinoculation and persist through the pustular stage of disease. To extend these observations to the ulcerative stage of disease, and to compare results in the human model with those of natural disease, we obtained biopsies from patients with naturally acquired chancroid. All ulcers were culture positive for H. ducreyi and histologically very similar to pustules from the human model. Staining with H. ducreyi-specific monoclonal antibodies demonstrated H. ducreyi within 5 biopsies. The organism was chiefly found within the granulocytic infiltrate of the ulcer. Dual staining for H. ducreyi and eukaryotic tissue components showed that H. ducreyi colocalized with neutrophils and fibrin at the ulcerative stage of disease. No bacteria were associated with keratinocytes, fibroblasts, or collagen. Overall, these findings are consistent with results from the human model. This is the first reported study to localize bacteria specifically identified as H. ducreyi within naturally acquired chancroid.

Localization of Haemophilus ducreyi in naturally acquired chancroidal ulcers. Publishing Authors By Initials

ME Bauer,CA Townsend,AR Ronald,SM Spinola,

For similar pathological conditions, signs and symptoms: pathologic processes: ulcer research abstracts see: pathological conditions, signs and symptoms: pathologic processes: ulcer research

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Localization of Haemophilus ducreyi in naturally acquired chancroidal ulcers. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Microbes and infection / Institut Pasteur

VOLUME: 8

Page Numbers: 2465-8

Journal Abbreviation: Microbes Infect.

ISSN: 1286-4579

DAY: 25

MONTH: 07

YEAR: 2006

Localization of Haemophilus ducreyi in naturally acquired chancroidal ulcers. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100883508

Localization of Haemophilus ducreyi in naturally acquired chancroidal ulcers. Keywords Mesh Terms:

KEYWORDS: Ulcer

MESH TERMS: microbiology

Chemical & Substance for Abstract: Localization of Haemophilus ducreyi in naturally acquired chancroidal ulcers. Information

Substance Name: Fibrin

Registry Number: 9001-31-4

Grant and Affiliation Information for Localization of Haemophilus ducreyi in naturally acquired chancroidal ulcers.

AFFILIATION: Department of Microbiology and Immunology, School of Medicine, Indiana University, 635 Barnhill Drive, Room MS 420, Indianapolis, IN 46202-5124, USA. mebauer@iupui.edu

Country: France

AGENCY: United States NIAID

GRANT: AI27863

ACRONYM: AI

MEDLINETA: Microbes Infect

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