Localization of 17-kDa myosin light chain isoforms in cultured aortic smooth muscle cells.

Localization of 17-kDa myosin light chain isoforms in cultured aortic smooth muscle cells. Research Abstract Details 

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Localization of 17-kDa myosin light chain isoforms in cultured aortic smooth muscle cells. Abstract Text:

K Takeuchi,S Senba,K Furukawa,M Eto,F Morita,

Smooth muscle myosin II contains two 17-kDa essential light chain isoforms (LC17gi and LC17nm) of which the relative contents differ among myosins. To understand the roles of LC17 isoforms in the functions of myosin, we performed an immunofluorescence microscopic examination of their localization in primary cultured cells isolated from rat aortic smooth muscle. To identify the isoforms, rabbit polyclonal antibodies were prepared against C-terminal nonapeptides corresponding to either LC17gi or LC17nm from porcine aortic smooth muscle myosin. These isoforms differ in only 5 amino acid residues within the C-terminal 9 residues. These antibodies specifically recognize each LC17 isoform on urea-PAGE of total rat aortic cell lysates. Immediately after plating, the smooth muscle cells stained heterogeneously with each antibody, indicating differing contents of LC17 isoforms among cells. On double staining 1-2 d cultures with both antibodies, LC17nm was detected diffusely throughout the cytoplasm, whereas LC17gi was concentrated in specific regions such as the cell periphery and the base of cytoplasmic processes. These results support the suggestion that myosin containing LC17gi is essential for force-generation by aortic smooth muscle and that myosin containing LC17nm may play an important role in maintaining smooth muscle tension.

Localization of 17-kDa myosin light chain isoforms in cultured aortic smooth muscle cells. Publishing Authors By Initials

K Takeuchi,S Senba,K Furukawa,M Eto,F Morita,

For similar biochemical phenomena, metabolism, and nutrition: metabolism: pharmacokinetics: tissue distribution research abstracts see: biochemical phenomena, metabolism, and nutrition: metabolism: pharmacokinetics: tissue distribution research

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Localization of 17-kDa myosin light chain isoforms in cultured aortic smooth muscle cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 125

Page Numbers: 334-42

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1999

Localization of 17-kDa myosin light chain isoforms in cultured aortic smooth muscle cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Localization of 17-kDa myosin light chain isoforms in cultured aortic smooth muscle cells. Keywords Mesh Terms:

KEYWORDS: Tissue Distribution

MESH TERMS: metabolism

Chemical & Substance for Abstract: Localization of 17-kDa myosin light chain isoforms in cultured aortic smooth muscle cells. Information

Substance Name: Protein Isoforms

Registry Number: 0

Grant and Affiliation Information for Localization of 17-kDa myosin light chain isoforms in cultured aortic smooth muscle cells.

AFFILIATION: Osaka Prefectural College of Health Science, Habikino, Osaka, 583-0872, Japan.

Country: JAPAN

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MEDLINETA: J Biochem

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