Localization and characterization of protein-protein interaction sites.

Localization and characterization of protein-protein interaction sites. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Localization and characterization of protein-protein interaction sites. Abstract Text:

Chingakham Ranjit Singh,Katsura Asano,

This chapter aims to describe methods to identify and characterize protein-protein interactions that were developed during our studies on translation initiation factor complexes. Methods include the two-hybrid assay, the GST pull-down assay, and the coimmunoprecipitation (co-IP) assay. The two-hybrid assay provides for a convenient start to find the minimal interaction domains, which generally produce well-behaved recombinant proteins suited for various in vitro interaction assays. Emphasis is placed on demonstrating physiological relevance of identified interactions. The effective strategy is to find mutations that reduce the interaction by genetic or site-directed mutational approaches and obtain correlations between their effects in vitro (GST pull down) and effects in vivo (co-IP).

Localization and characterization of protein-protein interaction sites. Publishing Authors By Initials

CR Singh,K Asano,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Localization and characterization of protein-protein interaction sites. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Methods in enzymology

VOLUME: 429

Page Numbers: 139-61

Journal Abbreviation: Meth. Enzymol.

ISSN: 0076-6879

DAY: 3

MONTH: 12

YEAR: 2007

Localization and characterization of protein-protein interaction sites. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 212271

Localization and characterization of protein-protein interaction sites. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Localization and characterization of protein-protein interaction sites. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Localization and characterization of protein-protein interaction sites.

AFFILIATION: Molecular, Cellular, and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, Kansas, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM64781

ACRONYM: GM

MEDLINETA: Methods Enzymol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Localization and characterization of protein-protein interaction sites Related Publications

• A novel DNA binding protein that recognizes the methylated c-Myc binding motif.
• Adenosine 3' : 5'-monophosphate binding activity and guanosine 3' : 5'-monophosphate binding activity during adrenocorticotropic hormone stimulation in rat adrenal gland.
• Age dependency of mitochondrial DNA decrease differs in different tissues of rat.
• Amino sugar metabolism in the silkworm, Bombyx mori.
• Analysis of human IgA nephropathy-like disease in galactosyltransferase KO mice.
• Ascorbate synthesizing system in rat liver microsomes. I. Gulonolactone-reducible pigment as a prosthetic group of gulonolactone oxidase.
• Carbohydrate content of fibroin and sericin of the silkworm, Bombyx mori.
• Carboxy-terminal modulator protein induces Akt phosphorylation and activation, thereby enhancing antiapoptotic, glycogen synthetic, and glucose uptake pathways.
• Diastereomer-specific effects of double-stranded peptides conjugated with -L-Tyr-L-Phe- or -L-Tyr-D-Phe- residues on tyrosine phosphorylation and inhibition of src(ts)NRK, A431, MCF-7, and DU145 cell growth.
• Effects of antibiotics on adaptive formation of mitochondria in yeast.
• Enhancement of stress-induced synthesis of stress proteins by mastoparan in C6 rat glioma cells.
• Establishment and characterization of a colonic epithelial cell line MCE301 from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.
• Eukaryotic Initiation Factor (eIF) 1 Carries Two Distinct eIF5-binding Faces Important for Multifactor Assembly and AUG Selection.
• G protein gamma subunits coimmunoprecipitated with antibodies against alpha subunits: identification of major isoforms in cultured cells by silver stain and immunoblotting with conventional transfer procedure.
• Hyponatremic seizure associated with acute respiratory infection.
• Immunohistochemical expression of T, Tn and sialyl-Tn antigens and clinical outcome in human breast carcinoma.
• Immunolocalization of farnesoid X receptor (FXR) in mouse tissues using tissue microarray.
• Impact of ABO incompatibility on engraftment and transfusion requirement after unrelated cord blood transplantation: a single institute experience in Japan.
• Involvement of Gq/11 in both integrin signal-dependent and -independent pathways regulating endothelin-induced neural progenitor proliferation.
• Localization and characterization of protein-protein interaction sites.
• Regulation of gut-derived resistin-like molecule beta expression by nutrients.
• Rheological studies on calcium-sensitive gelation of actin filaments by actinogelin.
• Shielding design calculation for SPring-8 beamlines using STAC8.
• Stable expression of gastric proton pump activity at the cell surface.
• Structural requirements for hemolytic activity of F-glycoprotein of HVJ (Sendai virus) studied by proteolytic dissection.
• Temporally distinctive changes of alternative splicing patterns during myogenic differentiation of C2C12 cells.
• Transrectal high-intensity focused ultrasound for treatment for patients with biochemical failure after radical prostatectomy.
• Unusual variant of persistent primitive hypoglossal artery.
• Yeast phenotypic assays on translational control.
• differential effect of Listeria monocytogenes infection on cytokine production and cytotoxicity of CD8 T cells.