Local function of urokinase receptor at the adhesion contact sites of a metastatic tumor cell.

Local function of urokinase receptor at the adhesion contact sites of a metastatic tumor cell. Research Abstract Details 

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Local function of urokinase receptor at the adhesion contact sites of a metastatic tumor cell. Abstract Text:

The local activity of urokinase and its receptor associated with a cloned cell (C5) obtained from the cloning of Detroit 562 was investigated. The cellular binding sites, similar structure to adhesion plaques, were visualized by fluorescein labeled urokinase and the number was determined to be 300 per cell. The binding sites for radioiodinated urokinase were found to be 30 thousand per cell. Thus, about as many as 100 receptor molecules per site was estimated to be associated with the cellular membrane domains. Immunofluorescence studies demonstrated that the receptors were colocalized with a set of adhesion and cytoskeletal proteins such as vinculin, alpha-actinin and actin; localizing at the adhesion sites. These proteins soluble in 9 M urea were able to be reconstituted by dialyzing out the urea against low ionic buffer solution. It was demonstrated that vinculin and actin were co-associated. Since cell bound urokinase revealed fibrinolytic activity, it was suggested that the focal adhesions of the migrating cells would facilitate proteolytic action when cells move across the matrix architectures.

Local function of urokinase receptor at the adhesion contact sites of a metastatic tumor cell. Publishing Authors By Initials

For similar proteins: cytoskeletal proteins: vinculin research abstracts see: proteins: cytoskeletal proteins: vinculin research

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Local function of urokinase receptor at the adhesion contact sites of a metastatic tumor cell. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Thrombosis research. Supplement

VOLUME: 10

Page Numbers: 55-61

Journal Abbreviation: Thromb. Res. Suppl.

ISSN: 0049-3848

DAY: 28

MONTH: 02

YEAR: 1990

Local function of urokinase receptor at the adhesion contact sites of a metastatic tumor cell. Information

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LANGUAGE: eng

NlmUniqueID: 8309239

Local function of urokinase receptor at the adhesion contact sites of a metastatic tumor cell. Keywords Mesh Terms:

KEYWORDS: Vinculin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Local function of urokinase receptor at the adhesion contact sites of a metastatic tumor cell. Information

Substance Name: Urinary Plasminogen Activator

Registry Number: EC 3.4.21.73

Grant and Affiliation Information for Local function of urokinase receptor at the adhesion contact sites of a metastatic tumor cell.

AFFILIATION: Department of Physiology, Shimane Medical University, Izumo, Japan.

Country: UNITED STATES

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MEDLINETA: Thromb Res Suppl

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