Liquid chromatographic analysis of vitamin B6 in reconstituted infant formula: collaborative study.

Liquid chromatographic analysis of vitamin B6 in reconstituted infant formula: collaborative study. Research Abstract Details 

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Liquid chromatographic analysis of vitamin B6 in reconstituted infant formula: collaborative study. Abstract Text:

A liquid chromatographic (LC) method was validated for the determination of total vitamin B6 in infant formula. Total vitamin B6 was quantified by converting the phosphorylated and free vitamers into pyridoxine. Pyridoxine was determined by ion pair reversed-phase LC with fluorescence detection. The method was subjected to an AOAC collaborative study involving a factory-manufactured, milk- and soy-based infant formula. Each was spiked at 3 concentrations in the range of 0-1 microg/g and sent as blind duplicate to participant laboratories. Nine laboratories returned valid data which were statistically analyzed for outliers and precision parameters. The repeatability relative standard deviation (RSD(r)) ranges were 2.0-4.0 and 3.5-5.9% for fortified milk- and soy-based formulas, respectively. The reproducibility relative standard deviation (RSD(R)) ranges were 8.2-8.4 and 6.7-11.2% for fortified milk- and soy-based formulas, respectively. HORRAT values ranged from 0.42 to 0.53, indicating that the precision of the method is acceptable. The mean RSD(r):RSD(R) values were 0.60 and 0.55 for milk- and soy-based formulas, respectively. As expected, RSDs for the unfortified samples were higher, but their HORRAT values (0.81 and 2.06) helped define a realistic limit of quantitation as 0.05 microg/g. Recovery data were quantitative and varied between 81.4 and 98.0% (mean = 89.8%) for each of 6 spiked materials.

Liquid chromatographic analysis of vitamin B6 in reconstituted infant formula: collaborative study. Publishing Authors By Initials

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: pyridines: picolines: vitamin b 6 research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: pyridines: picolines: vitamin b 6 research

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Liquid chromatographic analysis of vitamin B6 in reconstituted infant formula: collaborative study. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of AOAC International

VOLUME: 88

Page Numbers: 30-7

Journal Abbreviation: J AOAC Int

ISSN: 1060-3271

DAY: 17

MONTH: 03

YEAR: 2008

Liquid chromatographic analysis of vitamin B6 in reconstituted infant formula: collaborative study. Information

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LANGUAGE: eng

NlmUniqueID: 9215446

Liquid chromatographic analysis of vitamin B6 in reconstituted infant formula: collaborative study. Keywords Mesh Terms:

KEYWORDS: Vitamin B 6

MESH TERMS: metabolism

Chemical & Substance for Abstract: Liquid chromatographic analysis of vitamin B6 in reconstituted infant formula: collaborative study. Information

Substance Name: Vitamin B 6

Registry Number: 8059-24-3

Grant and Affiliation Information for Liquid chromatographic analysis of vitamin B6 in reconstituted infant formula: collaborative study.

AFFILIATION: U.S. Food and Drug Administration, Southeast Regional Laboratory, 60 Eighth St, NE, Atlanta, GA 30309, USA.

Country: United States

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MEDLINETA: J AOAC Int

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