Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA.

Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA. Research Abstract Details 

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Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA. Abstract Text:

Rumiana Koynova,Yury S Tarahovsky,Li Wang,Robert C MacDonald,

Lipoplexes containing a mixture of cationic phospholipids dioleoylethylphosphatidylcholine (EDOPC) and dilauroylethylphosphatidylcholine (EDLPC) are known to be far more efficient agents in transfection of cultured primary endothelial cells than are lipoplexes containing either lipid alone. The large magnitude of the synergy permits comparison of the physical and physico-chemical properties of lipoplexes that have very different transfection efficiencies, but minor chemical differences. Here we report that the superior transfection efficiency of the EDLPC/EDOPC lipoplexes correlates with higher surface activity, higher affinity to interact and mix with negatively charged membrane-mimicking liposomes, and with considerably more efficient DNA release relative to the EDOPC lipoplexes. Observations on cultured cells agree with the results obtained with model systems; confocal microscopy of transfected human umbilical artery endothelial cells (HUAEC) demonstrated more extensive DNA release into the cytoplasm and nucleoplasm for the EDLPC/EDOPC lipoplexes than for EDOPC lipoplexes; electron microscopy of cells fixed and embedded directly on the culture dish revealed contact of EDLPC/EDOPC lipoplexes with various cellular membranes, including those of the endoplasmic reticulum, mitochondria and nucleus. The sequence of events outlining efficient lipofection is discussed based on the presented data.

Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA. Publishing Authors By Initials

R Koynova,YS Tarahovsky,L Wang,RC MacDonald,

For similar cardiovascular system: blood vessels: arteries: umbilical arteries research abstracts see: cardiovascular system: blood vessels: arteries: umbilical arteries research

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Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Biochimica et biophysica acta

VOLUME: 1768

Page Numbers: 375-86

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 1

MONTH: 11

YEAR: 2006

Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA. Information

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LANGUAGE: eng

NlmUniqueID: 217513

Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA. Keywords Mesh Terms:

KEYWORDS: Umbilical Arteries

MESH TERMS: metabolism

Chemical & Substance for Abstract: Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA.

AFFILIATION: Department of Biochemistry, Molecular Biology and Cell Biology Northwestern University 2205 Tech Drive, Evanston, IL 60208, USA. r-tenchova@northwestern.edu

Country: Netherlands

AGENCY: United States NCRR

GRANT: RR08630

ACRONYM: RR

MEDLINETA: Biochim Biophys Acta

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