LINE-1 Hypomethylation in a Choline-Deficiency-Induced Liver Cancer in Rats: Dependence on Feeding Period.

LINE-1 Hypomethylation in a Choline-Deficiency-Induced Liver Cancer in Rats: Dependence on Feeding Period. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

LINE-1 Hypomethylation in a Choline-Deficiency-Induced Liver Cancer in Rats: Dependence on Feeding Period. Abstract Text:

Kiyoshi Asada,Yashige Kotake,Rumiko Asada,Deborah Saunders,Robert H Broyles,Rheal A Towner,Hiroshi Fukui,Robert A Floyd,

Chronic feeding of methyl-donor (methionine, choline, folic acid, and vitamin B12) deficient diet induces hepatocellular carcinoma formation in rats. Previous studies have shown that promoter CpG islands in various cancer-related genes are aberrantly methylated in this model. Moreover, the global genome in methyl-donor-deficient diet fed rats contains a lesser amount of 5-methylcytosine than control livers. It is speculated that more than 90% of all 5-methylcytosines lie within the CpG islands of the transposons, including the long/short interspersed nucleotide elements (LINE and SINE). It is considered that the 5-methylcytosines in LINE-1 limit the ability of retrotransposons to be activated and transcribed; therefore, the extent of hypomethylation of LINE-1 could be a surrogate marker for aberrant methylation in other tumor-related genes as well as genome instability. Additionally, LINE-1 methylation status has been shown to be a good indicator of genome-wide methylation. In this study, we determined cytosine methylation status in the LINE-1 repetitive sequences of rats fed a choline-deficient (CD) diet for various durations and compared these with rats fed a choline-sufficient (CS) diet. The methylation status of LINE-1 was assessed by the combined bisulfite restriction analysis (COBRA) method, where the amount of bisulfite-modified and RsaI-cleaved DNA was quantified using gel electrophoresis. Progressive hypomethylation was observed in LINE-1 of CD livers as a function of feeding time; that is, the amount of cytosine in total cytosine (methylated and unmethylated) increased from 11.1% (1 week) to 19.3% (56 weeks), whereas in the control CS livers, it increased from 9.2% to 12.9%. Hypomethylation in tumor tissues was slightly higher (6%) than the nontumorous surrounding tissue. The present result also indicates that age is a factor influencing the extent of cytosine methylation.

LINE-1 Hypomethylation in a Choline-Deficiency-Induced Liver Cancer in Rats: Dependence on Feeding Period. Publishing Authors By Initials

K Asada,Y Kotake,R Asada,D Saunders,RH Broyles,RA Towner,H Fukui,RA Floyd,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

LINE-1 Hypomethylation in a Choline-Deficiency-Induced Liver Cancer in Rats: Dependence on Feeding Period. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomedicine & biotechnology

VOLUME: 2006

Page Numbers: 17142

Journal Abbreviation:

ISSN: 1110-7243

DAY: 31

MONTH: 07

YEAR: 2006

LINE-1 Hypomethylation in a Choline-Deficiency-Induced Liver Cancer in Rats: Dependence on Feeding Period. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101135740

LINE-1 Hypomethylation in a Choline-Deficiency-Induced Liver Cancer in Rats: Dependence on Feeding Period. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: LINE-1 Hypomethylation in a Choline-Deficiency-Induced Liver Cancer in Rats: Dependence on Feeding Period. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for LINE-1 Hypomethylation in a Choline-Deficiency-Induced Liver Cancer in Rats: Dependence on Feeding Period.

AFFILIATION:

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biomed Biotechnol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

LINE-1 Hypomethylation in a Choline-Deficiency-Induced Liver Cancer in Rats: Dependence on Feeding Period Related Publications

• A descriptive account of New Zealand mothers' responses to open-ended questions on their breast feeding experiences.
• A year's experience with a mobile coronary resuscitation unit.
• Acute pancreatitis and the rational therapy.
• An artificial neural network for SPECT image reconstruction.
• Anaerobic digestion of high-strength cheese whey utilizing semicontinuous digesters and chemical flocculant addition.
• Breast cancer detection using neutron stimulated emission computed tomography: prominent elements and dose requirements.
• Care service in general practice: a two-year survey.
• Changing clinical picture of neurosyphilis: report of seven unusual cases.
• Clinal variation in Chinese height and weight: Evidence from the descendants of emigrants to Taiwan.
• Clinical symptoms of schizophrenia affect reference-independent measures of task-induced EEG alpha asymmetry.
• Cutaneous lesions due to Mycobacterium kansasii.
• Doctors on the move.
• Drug utilization and prescribing patterns in a skilled nursing facility: the need for a rational approach to therapeutics.
• Effectiveness of an intervention for disseminating Cochrane reviews to nurses.
• Etiological factors in pancreatitis.
• Evaluation of information-theoretic similarity measures for content-based retrieval and detection of masses in mammograms.
• Fatigue; Ergonomics Research Society's symposium.
• Hyperemic flow heterogeneity within the calf, foot, and forearm measured with continuous arterial spin labeling MRI.
• Is the measurement of angles on radiographs accurate? Brief report.
• LINE-1 Hypomethylation in a Choline-Deficiency-Induced Liver Cancer in Rats: Dependence on Feeding Period.
• Medical malpractice: trends in litigation.
• Methodologic issues in exercise intervention research in oncology.
• On the death of Kentuckian Charles Floyd, August 20, 1804--the only casualty of the Lewis and Clark expedition: a solution to a 200-year-old medical mystery.
• Patterning and polarity in seed plant shoots.
• Perspective: the origin of flowering plants and their reproductive biology--a tale of two phylogenies.
• Predictors of online information seeking by international students when disaster strikes their countries.
• Prevalence and correlates of hepatitis A among adult drug users: the significance of incarceration and race/ethnicity.
• Respiratory sensitization of a food manufacturing worker to konjac glucomannan.
• The treatment of acute pancreatitis, with discussion of mechanism of production, clinical manifestations and diagnosis and report of four cases.
• Use of Parks, Trails, Open Spaces, and Physical Activity Resources in Diverse Urban Communities: 245: 3:45 PM-4:10 PM.