Light-induced depolarization of neurons using a modified Shaker K(+) channel and a molecular photoswitch.

Light-induced depolarization of neurons using a modified Shaker K(+) channel and a molecular photoswitch. Research Abstract Details 

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Light-induced depolarization of neurons using a modified Shaker K(+) channel and a molecular photoswitch. Abstract Text:

James J Chambers,Matthew R Banghart,Dirk Trauner,Richard H Kramer,

To trigger action potentials in neurons, most investigators use electrical or chemical stimulation. Here we describe an optical stimulation method based on semi-synthetic light-activated ion channels. These SPARK (synthetic photoisomerizable azobenzene-regulated K(+)) channels consist of a synthetic azobenzene-containing photoswitch and a genetically modified Shaker K(+) channel protein. SPARK channels with a wild-type selectivity filter elicit hyperpolarization and suppress action potential firing when activated by 390 nm light. A mutation in the pore converts the K(+)-selective Shaker channel into a nonselective cation channel. Activation of this modified channel with the same wavelength of light elicits depolarization of the membrane potential. Expression of these depolarizing SPARK channels in neurons allows light to rapidly and reversibly trigger action potential firing. Hence, hyper- and depolarizing SPARK channels provide a means for eliciting opposite effects on neurons in response to the same light stimulus.

Light-induced depolarization of neurons using a modified Shaker K(+) channel and a molecular photoswitch. Publishing Authors By Initials

JJ Chambers,MR Banghart,D Trauner,RH Kramer,

For similar proteins: carrier proteins: membrane transport proteins: ion channels: potassium channels: potassium channels, voltage-gated: shaker superfamily of potassium channels research abstracts see: proteins: carrier proteins: membrane transport proteins: ion channels: potassium channels: potassium channels, voltage-gated: shaker superfamily of potassium channels research

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MEDLINE DATE:

Light-induced depolarization of neurons using a modified Shaker K(+) channel and a molecular photoswitch. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of neurophysiology

VOLUME: 96

Page Numbers: 2792-6

Journal Abbreviation: J. Neurophysiol.

ISSN: 0022-3077

DAY: 26

MONTH: 07

YEAR: 2006

Light-induced depolarization of neurons using a modified Shaker K(+) channel and a molecular photoswitch. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375404

Light-induced depolarization of neurons using a modified Shaker K(+) channel and a molecular photoswitch. Keywords Mesh Terms:

KEYWORDS: Shaker Superfamily of Potassium Channels

MESH TERMS: radiation effects

Chemical & Substance for Abstract: Light-induced depolarization of neurons using a modified Shaker K(+) channel and a molecular photoswitch. Information

Substance Name: maleimide

Registry Number: 541-59-3

Grant and Affiliation Information for Light-induced depolarization of neurons using a modified Shaker K(+) channel and a molecular photoswitch.

AFFILIATION: Department of Molecular and Cell Biology, University of California, Berkley, CA 94720, USA.

Country: United States

AGENCY: United States NEI

GRANT: EY-016249

ACRONYM: EY

MEDLINETA: J Neurophysiol

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