Levistolide A overcomes P-glycoprotein-mediated drug resistance in human breast carcinoma cells.

Levistolide A overcomes P-glycoprotein-mediated drug resistance in human breast carcinoma cells. Research Abstract Details 

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Levistolide A overcomes P-glycoprotein-mediated drug resistance in human breast carcinoma cells. Abstract Text:

Fei Chen,Tao Wang,Jia Wang,Zi-Qiang Wang,Ming Qian,

Aim: The aim of the present study was to investigate the reversing effect of levistolide A (LA) on P-glycoprotein (P-gp)-mediated multidrug resistance (MDR) in human breast carcinoma Bcap37/MDR1 cells. Methods: After chemotherapeutic drugs (adriamycin or vincristine) used alone or in combination with LA, cell proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and cell cycle distribution by flow cytometry. RT-PCR was used to detect MDR1 gene transcription and the Western blot assay was used to assess P-gp expression and the cleavages of poly(ADP-ribose) polymerase and caspase-3. Apoptosis was detected by terminal transferase-mediated dUTP nick end-labeling assay. Moreover, the P-gp function was evaluated by the intracellular accumulation of the P-gp substrate detected by flow cytometry. Results: We found the subcytotoxic doses of LA significantly enhanced adriamycin- or vincristine- induced G2/M arrest and apoptosis. These effects were consistent with the ability of LA to inhibit P-gp function. Moreover, LA dramatically enhanced the verapamil (VER) ability to reverse drug resistance. Conclusion: LA has the potential to be developed as a novel P-gp modulator. Furthermore, the combination of LA and VER might represent a more sufficient but less toxic anti-MDR regimen.

Levistolide A overcomes P-glycoprotein-mediated drug resistance in human breast carcinoma cells. Publishing Authors By Initials

F Chen,T Wang,J Wang,ZQ Wang,M Qian,

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Levistolide A overcomes P-glycoprotein-mediated drug resistance in human breast carcinoma cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta pharmacologica Sinica

VOLUME: 29

Page Numbers: 458-64

Journal Abbreviation: Acta Pharmacol. Sin.

ISSN: 1671-4083

DAY: 24

MONTH: Apr

YEAR: 2008

Levistolide A overcomes P-glycoprotein-mediated drug resistance in human breast carcinoma cells. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Levistolide A overcomes P-glycoprotein-mediated drug resistance in human breast carcinoma cells.

AFFILIATION: National Laboratory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiaotong University, Shanghai 200032, China. faithchen@yahoo.com.

Country: China

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MEDLINETA: Acta Pharmacol Sin

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