Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis.

Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis. Research Abstract Details 

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Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis. Abstract Text:

Julio Croda, Ramos,James Matsunaga,Adriano Queiroz,Akira Homma,Lee W Riley,David A Haake,Mitermayer G Reis,Albert I Ko,

There is an urgent need for improved diagnosis of leptospirosis, an emerging infectious disease which imparts a large disease burden in developing countries. We evaluated the use of Leptospira immunoglobulin (Ig)-like (Lig) proteins as a serodiagnostic marker for leptospirosis. Lig proteins have bacterial immunoglobulin-like (Big) tandem repeat domains, a moiety found in virulence factors in other pathogens. Sera from patients identified during urban outbreaks in Brazil reacted strongly with immunoblots of a recombinant fragment comprised of the second to sixth Big domains of LigB from L. interrogans serovar Copenhageni, the principal agent for transmission in this setting. Furthermore, the sera recognized an analogous LigB fragment derived from L. kirschneri serovar Grippotyphosa, a pathogenic serovar which is not endemic to the study area. The immunoblot assay detected anti-LigB IgM antibodies in sera from 92% (95% confidence interval, 85 to 96%) of patients during acute-phase leptospirosis. The assay had a sensitivity of 81% for sera from patients with less than 7 days of illness. Anti-LigB antibodies were found in sera from 57% of the patients who did not have detectable anti-whole-Leptospira responses as detected by IgM enzyme-linked immunosorbent assay and microagglutination test. The specificities of the assay were 93 to 100% and 90 to 97% among sera from healthy individuals and patients with diseases that have clinical presentations that overlap with those of leptospirosis, respectively. These findings indicate that the antibody response to this putative virulence determinant is a sensitive and specific marker for acute infection. The use of this marker may aid the prompt and timely diagnosis required to reduce the high mortality associated with severe forms of the disease.

Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis. Publishing Authors By Initials

J Croda,JG Ramos,J Matsunaga,A Queiroz,A Homma,LW Riley,DA Haake,MG Reis,AI Ko,

For similar bacterial infections and mycoses: bacterial infections: gram-negative bacterial infections: leptospirosis research abstracts see: bacterial infections and mycoses: bacterial infections: gram-negative bacterial infections: leptospirosis research

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MEDLINE DATE:

Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of clinical microbiology

VOLUME: 45

Page Numbers: 1528-34

Journal Abbreviation:

ISSN: 0095-1137

DAY: 14

MONTH: 03

YEAR: 2007

Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505564

Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis. Keywords Mesh Terms:

KEYWORDS: Leptospirosis

MESH TERMS: epidemiology

Chemical & Substance for Abstract: Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis. Information

Substance Name: Immunoglobulins

Registry Number: 0

Grant and Affiliation Information for Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis.

AFFILIATION: Gonçalo Moniz Research Center, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Brazil.

Country: United States

AGENCY: United States FIC

GRANT: TW-00919

ACRONYM: TW

MEDLINETA: J Clin Microbiol

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