Leptin regulates gallbladder genes related to absorption and secretion.

Leptin regulates gallbladder genes related to absorption and secretion. Research Abstract Details 

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Leptin regulates gallbladder genes related to absorption and secretion. Abstract Text:

Deborah A Swartz-Basile,Debao Lu,David P Basile,Shannon J Graewin,Hayder Al-Azzawi,James M Kiely,Abhishek Mathur,Kyle Yancey,Henry A Pitt,

Dysregulation of gallbladder ion and water absorption and/or secretion has been linked to cholesterol crystal and gallstone formation. We have recently demonstrated that obese, leptin-deficient (Lep(ob)) mice have enlarged gallbladder volumes and decreased gallbladder contractility and that leptin administration to these mice normalizes gallbladder function. However, the effect of leptin on gallbladder absorption/secretion is not known. Therefore, we sought to determine whether leptin would alter the expression of genes involved in water and ion transport across the gallbladder epithelium. Affymetrix oligonucleotide microarrays representing 39,000 transcripts were used to compare gallbladder gene-expression profiles from 12-wk-old control saline-treated Lep(ob) and from leptin-treated Lep(ob) female mice. Leptin administration to Lep(ob) mice decreased gallbladder volume, bile sodium concentration, and pH. Leptin repletion upregulated the expression of aquaporin 1 water channel by 1.3-fold and downregulated aquaporin 4 by 2.3-fold. A number of genes involved in sodium transport were also influenced by leptin replacement. Epithelial sodium channel-alpha and sodium hydrogen exchangers 1 and 3 were moderately downregulated by 2.0-, 1.6-, and 1.3-fold, respectively. Carbonic anhydrase-IV, which plays a role in the acidification of bile, was upregulated 3.7-fold. In addition, a number of inflammatory cytokines that are known to influence gallbladder epithelial cell absorption and secretion were upregulated. Thus leptin, an adipocyte-derived cytokine involved with satiety and energy balance, influences gallbladder bile volume, sodium, and pH as well as multiple inflammatory cytokine genes and genes related to water, sodium, chloride, and bicarbonate transport.

Leptin regulates gallbladder genes related to absorption and secretion. Publishing Authors By Initials

DA Swartz-Basile,D Lu,DP Basile,SJ Graewin,H Al-Azzawi,JM Kiely,A Mathur,K Yancey,HA Pitt,

For similar genetic processes: gene expression regulation: up-regulation research abstracts see: genetic processes: gene expression regulation: up-regulation research

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Leptin regulates gallbladder genes related to absorption and secretion. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Gastrointestinal a

VOLUME: 293

Page Numbers: G84-90

Journal Abbreviation: Am. J. Physiol. Gastrointest.

ISSN: 0193-1857

DAY: 26

MONTH: 04

YEAR: 2007

Leptin regulates gallbladder genes related to absorption and secretion. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901227

Leptin regulates gallbladder genes related to absorption and secretion. Keywords Mesh Terms:

KEYWORDS: Up-Regulation

MESH TERMS: metabolism

Chemical & Substance for Abstract: Leptin regulates gallbladder genes related to absorption and secretion. Information

Substance Name: Sodium

Registry Number: 7440-23-5

Grant and Affiliation Information for Leptin regulates gallbladder genes related to absorption and secretion.

AFFILIATION: Department of Surgery, Indiana University School of Medicine, 535 Barnhill Drive, RT 130D, Indianapolis, IN 46202, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK 44279-09

ACRONYM: DK

MEDLINETA: Am J Physiol Gastrointest Live

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