Leptin acts as a mitogenic and antiapoptotic factor for colonic cancer cells.

Leptin acts as a mitogenic and antiapoptotic factor for colonic cancer cells. Research Abstract Details 

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Leptin acts as a mitogenic and antiapoptotic factor for colonic cancer cells. Abstract Text:

M R Hoda,S J Keely,L S Bertelsen,W G Junger,D Dharmasena,K E Barrett,

BACKGROUND: Obesity is associated with increased levels of leptin. The mitogenic actions of leptin have been identified in various cell types. Because obesity may be a risk factor for colonic cancer, the proliferative and antiapoptotic effects of leptin on colonic cancer cells and the role of mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3-K) signalling were investigated. METHODS: Three human colonic cancer cell lines (T(84), HT29/Cl.19A and Caco-2) were treated with leptin. Cell proliferation was measured using the XTT colorimetric assay and apoptosis by a cell death enzyme-linked immunosorbent assay. Inhibitors of MAPK and PI3-K were used to evaluate the role of these signalling pathways. Phosphorylation of the downstream components extracellular signal-regulated kinase (ERK) 1/2 and Akt was detected by western blotting. RESULTS: Leptin increased cell number in all cell lines in a dose-dependent manner and reduced the number of apoptotic cells in a cell line-dependent manner. Leptin also caused ERK1/2 and Akt phosphorylation. Pretreatment with inhibitors of MAPK and PI3-K inhibited these responses, attenuated the mitogenic action of leptin and abolished its antiapoptotic effects. CONCLUSION: Chronic increases in leptin concentration may enhance the growth of colonic cancers via MAPK and PI3-K pathways. These effects of leptin could provide a link between obesity and colonic cancer, and may represent a target for anticancer drug development.

Leptin acts as a mitogenic and antiapoptotic factor for colonic cancer cells. Publishing Authors By Initials

MR Hoda,SJ Keely,LS Bertelsen,WG Junger,D Dharmasena,KE Barrett,

For similar cells: cells, cultured: tumor cells, cultured research abstracts see: cells: cells, cultured: tumor cells, cultured research

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Leptin acts as a mitogenic and antiapoptotic factor for colonic cancer cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The British journal of surgery

VOLUME: 94

Page Numbers: 346-54

Journal Abbreviation:

ISSN: 0007-1323

DAY: 3

MONTH: Mar

YEAR: 2007

Leptin acts as a mitogenic and antiapoptotic factor for colonic cancer cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372553

Leptin acts as a mitogenic and antiapoptotic factor for colonic cancer cells. Keywords Mesh Terms:

KEYWORDS: Tumor Cells, Cultured

MESH TERMS: complications

Chemical & Substance for Abstract: Leptin acts as a mitogenic and antiapoptotic factor for colonic cancer cells. Information

Substance Name: Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Leptin acts as a mitogenic and antiapoptotic factor for colonic cancer cells.

AFFILIATION: Division of Gastroenterology, Department of Medicine, University of California, San Diego, School of Medicine, San Diego, California, USA.

Country: England

AGENCY: United States NIDDK

GRANT: DK28305

ACRONYM: DK

MEDLINETA: Br J Surg

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