Lentivirus-like particles without reverse transcriptase elicit efficient immune responses.

Lentivirus-like particles without reverse transcriptase elicit efficient immune responses. Research Abstract Details 

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Lentivirus-like particles without reverse transcriptase elicit efficient immune responses. Abstract Text:

Sean P McBurney,Kelly R Young,Casmiar I Nwaigwe,Adam C Soloff,Kelly Stefano Cole,Ted M Ross,

Following infection by HIV or SIV, reverse transcriptase (RT) directs the conversion of the single-stranded RNA genome into a double-stranded DNA molecule that integrates into the host cell genome. RT encodes for several immunogenic epitopes that are desirable for inclusion in a human vaccine for HIV infection, however, issues of safety have dampened enthusiasm for inclusion of an enzymatically-active RT molecule into an AIDS vaccine. In this study, virally-regulated, replication-incompetent lentiviral particles were expressed from DNA plasmids. The sequences for integrase, Vpr, Vif, Nef, and the long terminal repeats (LTRs) were deleted and mutations were engineered into capsid to decreases RNA packaging. Virus-like particles incorporated no RT (HIV-VLP DeltaRT or SHIV-VLP DeltaRT) or contained a full-length enzymatically-inactivated RT molecule (HIV-VLP or SHIV-VLP). Each secreted VLP was enveloped with a lipid bilayer derived from primate cells with embedded, native viral envelopes in similar concentrations as infectious virions. BALB/c mice were vaccinated (weeks 0, 3, and 6) with purified VLPs via intranasal inoculation in the presence of cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODNs). All VLPs, with or without RT, elicited both robust humoral and cellular immune responses to Gag, Pol, and Env antigens. Therefore, the lack of RT enhances the safety of these VLPs for use in future human clinical trials without a significant reduction in the overall immunogenicity of these VLP immunogens.

Lentivirus-like particles without reverse transcriptase elicit efficient immune responses. Publishing Authors By Initials

SP McBurney,KR Young,CI Nwaigwe,AC Soloff,KS Cole,TM Ross,

For similar viruses: virion research abstracts see: viruses: virion research

PUBMED ID PMID:

MEDLINE DATE:

Lentivirus-like particles without reverse transcriptase elicit efficient immune responses. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Current HIV research

VOLUME: 4

Page Numbers: 475-84

Journal Abbreviation: Curr. HIV Res.

ISSN: 1873-4251

DAY: 3

MONTH: Oct

YEAR: 2006

Lentivirus-like particles without reverse transcriptase elicit efficient immune responses. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101156990

Lentivirus-like particles without reverse transcriptase elicit efficient immune responses. Keywords Mesh Terms:

KEYWORDS: Virion

MESH TERMS: immunology

Chemical & Substance for Abstract: Lentivirus-like particles without reverse transcriptase elicit efficient immune responses. Information

Substance Name: RNA-Directed DNA Polymerase

Registry Number: EC 2.7.7.49

Grant and Affiliation Information for Lentivirus-like particles without reverse transcriptase elicit efficient immune responses.

AFFILIATION: Department of Medicine, Division of Infectious Diseases, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.

Country: Netherlands

AGENCY: United States NIAID

GRANT: AI51213

ACRONYM: AI

MEDLINETA: Curr HIV Res

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