Lead effects on development and function of bone marrow-derived dendritic cells promote Th2 immune responses.

Lead effects on development and function of bone marrow-derived dendritic cells promote Th2 immune responses. Research Abstract Details 

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Lead effects on development and function of bone marrow-derived dendritic cells promote Th2 immune responses. Abstract Text:

Donghong Gao,Tapan K Mondal,David A Lawrence,

Although lead (Pb) has significant effects on the development and function of macrophages, B cells, and T cells and has been suggested to promote allergic asthma in mice and humans, Pb modulation of bone marrow (BM)-derived dendritic cells (DCs) and the resultant DC effects on Th1 and Th2 development have not been examined. Accordingly, we cultured BM cells with murine granulocyte macrophage-colony stimulating factor (mGM-CSF)+/-PbCl(2). At day 10, culture supernatant (SN) and non-adherent cells were harvested for analysis. Additionally, day 10 non-adherent BM-DCs were harvested and recultured with mGM-CSF+LPS+/-Pb for 2 days. The day 10 Pb exposure significantly inhibited BM-DC generation, based on CD11c expression. Although fewer DCs were generated with Pb, the existing Pb-exposed DCs had significantly greater MHC-II expression than did the non-Pb-exposed DCs. However, these differences diminished upon LPS stimulation. After LPS stimulation, CD80, CD86, CD40, CD54, and MHC-II were all up-regulated on both Pb-DCs and DCs, but Pb-DCs expressed significantly less CD80 than did DCs. The CD86:CD80 ratio suggests a Pb-DC potential for Th2 cell development. After LPS stimulation, IL-6, IL-10, IL-12 (p70), and TNF-alpha levels significantly increased with both Pb-DCs and DCs, but Pb-DCs produced significantly less cytokines than did DCs, except for IL-10, which further supports Pb-DC preferential skewing toward type-2 immunity. In vitro studies confirm that Pb-DCs have the ability to polarize antigen-specific T cells to Th2 cells. Pb-DCs also enhanced allogeneic and autologous T cell proliferation in vitro, and in vivo studies suggested that Pb-DCs inhibited Th1 effects on humoral and cell-mediated immunity. The Pb effect was mainly on DCs, rather than on T cells, and Pb's modification of DC function appears to be the main cause of Pb's promotion of type-2-related immunity, which may relate to Pb's enhanced activation of the Erk/MAP kinase pathway.

Lead effects on development and function of bone marrow-derived dendritic cells promote Th2 immune responses. Publishing Authors By Initials

D Gao,TK Mondal,DA Lawrence,

For similar cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes: cd4-positive t-lymphocytes: t-lymphocytes, helper-inducer: th2 cells research abstracts see: cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes: cd4-positive t-lymphocytes: t-lymphocytes, helper-inducer: th2 cells research

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Lead effects on development and function of bone marrow-derived dendritic cells promote Th2 immune responses. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Toxicology and applied pharmacology

VOLUME: 222

Page Numbers: 69-79

Journal Abbreviation: Toxicol. Appl. Pharmacol.

ISSN: 0041-008X

DAY: 12

MONTH: 04

YEAR: 2007

Lead effects on development and function of bone marrow-derived dendritic cells promote Th2 immune responses. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 416575

Lead effects on development and function of bone marrow-derived dendritic cells promote Th2 immune responses. Keywords Mesh Terms:

KEYWORDS: Th2 Cells

MESH TERMS: immunology

Chemical & Substance for Abstract: Lead effects on development and function of bone marrow-derived dendritic cells promote Th2 immune responses. Information

Substance Name: Ovalbumin

Registry Number: 9006-59-1

Grant and Affiliation Information for Lead effects on development and function of bone marrow-derived dendritic cells promote Th2 immune responses.

AFFILIATION: Biggs Laboratory, Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201-0509, USA.

Country: United States

AGENCY: United States NIEHS

GRANT: ES11135

ACRONYM: ES

MEDLINETA: Toxicol Appl Pharmacol

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