LC3 and Autophagy.

LC3 and Autophagy. Research Abstract Details 

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LC3 and Autophagy. Abstract Text:

Microtubule-associated protein 1A/1B-light chain 3 (LC3) is a soluble protein with a molecular mass of approximately 17 kDa that is distributed ubiquitously in mammalian tissues and cultured cells. During autophagy, autophagosomes engulf cytoplasmic components, including cytosolic proteins and organelles. Concomitantly, a cytosolic form of LC3 (LC3-I) is conjugated to phosphatidylethanolamine to form LC3-phosphatidylethanolamine conjugate (LC3-II), which is recruited to autophagosomal membranes. Autophagosomes fuse with lysosomes to form autolysosomes, and intra-autophagosomal components are degraded by lysosomal hydrolases. At the same time, LC3-II in autolysosomal lumen is degraded. Thus, lysosomal turnover of the autophagosomal marker LC3-II reflects starvation-induced autophagic activity, and detecting LC3 by immunoblotting or immunofluorescence has become a reliable method for monitoring autophagy and autophagy-related processes, including autophagic cell death. Here we describe basic protocols to assay for endogenous LC3-II by immunoblotting, immunoprecipitation, and immunofluorescence.

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PUBLICATION TYPE: Journal Article

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 445

Page Numbers: 77-88

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 21

MONTH: 04

YEAR: 2008

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LANGUAGE: eng

NlmUniqueID: 9214969

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Grant and Affiliation Information for LC3 and Autophagy.

AFFILIATION: Department of Biochemistry, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan.

Country: United States

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MEDLINETA: Methods Mol Biol

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