LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells.

LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells. Abstract Text:

Seon Hwa Lee,Caterina Pettinella,Ian A Blair,

Reactive oxygen species (ROS) can mediate damage to cellular macromolecules and lipids. Lipid peroxidation is considered to be a major pathway by which ROS can cause tissue damage and alterations in cell membranes. Other factors affecting oxidative damage include the target molecules such as fatty acids, which are readily oxidized by ROS. Thus, lipid peroxidation may depend upon the cellular fatty acid composition. Analysis of saturated fatty acids that are present by liquid chromatography/mass spectrometry (LC/MS) is difficult because they are poorly ionized under electrospray ionization (ESI) conditions. The separation of short to very long chain saturated and unsaturated fatty acids is also very challenging when LC is employed instead of gas chromatography. The use of trimethylaminoethyl (TMAE) ester iodide derivatization has been shown previously to improve the sensitivities of saturated fatty acids in the ESI mode. A reversed-phase LC method using a diphenyl column was employed to separate 14 fatty acids as their TMAE derivatives. Stable isotope dilution LC/ESI/multiple reaction monitoring/MS methodology was then developed for the quantitative analysis of seven saturated and seven unsaturated forms of short (C14) to very long (C26) chain fatty acids as their TMAE ester iodide derivatives. This methodology has allowed the analysis of fatty acid composition from parental rat intestinal epithelial cell and rat intestinal epithelial cells transfected with cyclooxygenase-2, a model system of oxidative stress.

LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells. Publishing Authors By Initials

SH Lee,C Pettinella,IA Blair,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Current drug metabolism

VOLUME: 7

Page Numbers: 929-37

Journal Abbreviation: Curr. Drug Metab.

ISSN: 1389-2002

DAY: 3

MONTH: Dec

YEAR: 2006

LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100960533

LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Mass, Electrospray Ionizat

MESH TERMS: methods

Chemical & Substance for Abstract: LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells. Information

Substance Name: Ascorbic Acid

Registry Number: 50-81-7

Grant and Affiliation Information for LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells.

AFFILIATION: Center for Cancer Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6160, USA.

Country: Netherlands

AGENCY: United States NCI

GRANT: R01 CA95586

ACRONYM: CA

MEDLINETA: Curr Drug Metab

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells Related Publications

• 706.
• Accurate and efficient detection of pulmonary seed embolization in prostate iodine-125 permanent brachytherapy with a collimated gamma scintillation survey meter.
• An innovative, effective and cost effective survey method using a survey-check response format.
• Analysis of phosphorylation sites on focal adhesion kinase using nanospray liquid chromatography/multiple reaction monitoring mass spectrometry.
• Characterization of an etoposide-glutathione conjugate derived from metabolic activation by human cytochrome p450.
• Classifying Weight Status Using Body Mass Index and Percent Body Fat: 2130: Board #43 June 1 9:00 AM - 10:30 AM.
• Construct validity evidence for single-response items to estimate physical activity levels in large sample studies.
• Convenient and efficient syntheses of 4-hydroxy-2(E)-nonenal and 4-oxo-2(E)-nonenal.
• Coronary artery calcium, exercise tolerance, and CHD events in asymptomatic men.
• Death receptor-3 mediates apoptosis in human osteoblasts under narrowly regulated conditions.
• Does Faculty Practice Validate the NP Educator Role?
• Effect of a dose-response exercise study on daily step activity in postmenopausal women: 1558: board #48 may 30 3:30 PM - 5:00 PM.
• Effects of different doses of physical activity on cardiorespiratory fitness among sedentary, overweight or obese postmenopausal women with elevated blood pressure: a randomized controlled trial.
• Endogenous glutathione adducts.
• Estrogen receptor-beta modulates synthesis of bone matrix proteins in human osteoblast-like MG63 cells.
• Graded Doses of Exercise and Insulin Resistance in Nondiabetic Post-Menopausal Women: The DREW Study: 1540: Board #30 May 30 2:00 PM - 3:30 PM.
• Heightened sensitivity to facial expressions of emotion in borderline personality disorder.
• LC/ESI/MS analysis of saturated and unsaturated fatty acids in rat intestinal epithelial cells.
• Ml01 competence reviews in new zealand and the aftermath.
• Modulation of emotion by cognition and cognition by emotion.
• Mutational analysis of the flagellar protein FliG: sites of interaction with FliM and implications for organization of the switch complex.
• Occupation and breast cancer risk in Polish women: a population-based case-control study.
• Proteomics-based strategy to identify biomarkers and pharmacological targets in leukemias with t(4;11) translocations.
• Quantification of intracellular homocysteine by stable isotope dilution liquid chromatography/tandem mass spectrometry.
• Quantitative analysis of amyloid beta peptides in cerebrospinal fluid of Alzheimer's disease patients by immunoaffinity purification and stable isotope dilution liquid chromatography/negative electrospray ionization tandem mass spectrometry.
• Response options and expectations of reward in decision-making: the differential roles of dorsal and rostral anterior cingulate cortex.
• Subgroup Interactions and Changes in Cardiorespiratory Fitness among Postmenopausal Women: The DREW Study: 1556: Board #46 May 30 3:30 PM - 5:00 PM.
• Targeted quantitative analysis of fatty acids in atherosclerotic plaques by high sensitivity liquid chromatography/tandem mass spectrometry.
• The moving emission features in SS433 require a dynamical interpretation.
• [Anesthesia arrived unexpectedly and quickly; the anesthesiologist appeared more slowly. The story of the dissemination of the discovery of anesthesia in the United Kingdom and the United States of America.]