Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate.

Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate. Research Abstract Details 

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Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate. Abstract Text:

Jayakrishnan Nandakumar,Pravin A Nair,Stewart Shuman,

NAD(+)-dependent DNA ligases (LigA) are ubiquitous in bacteria and essential for growth. Their distinctive substrate specificity and domain organization vis-a-vis human ATP-dependent ligases make them outstanding targets for anti-infective drug discovery. We report here the 2.3 A crystal structure of Escherichia coli LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition. LigA envelopes the DNA within a protein clamp. Large protein domain movements and remodeling of the active site orchestrate progression through the three chemical steps of the ligation reaction. The structure inspires a strategy for inhibitor design.

Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate. Publishing Authors By Initials

J Nandakumar,PA Nair,S Shuman,

For similar biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research abstracts see: biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research

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Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular cell

VOLUME: 26

Page Numbers: 257-71

Journal Abbreviation:

ISSN: 1097-2765

DAY: 27

MONTH: Apr

YEAR: 2007

Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9802571

Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate. Keywords Mesh Terms:

KEYWORDS: Species Specificity

MESH TERMS: metabolism

Chemical & Substance for Abstract: Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate. Information

Substance Name: LigA protein, E coli

Registry Number: EC 6.5.1.2

Grant and Affiliation Information for Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate.

AFFILIATION: Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10021, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM63611

ACRONYM: GM

MEDLINETA: Mol Cell

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