Laser scanning confocal imaging of abnormal or arrested human preimplantation embryos.

Laser scanning confocal imaging of abnormal or arrested human preimplantation embryos. Research Abstract Details 

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Laser scanning confocal imaging of abnormal or arrested human preimplantation embryos. Abstract Text:

PURPOSE: The improved resolution and optical sectioning of a confocal microscope make it an ideal instrument for extracting three-dimensional information, especially from extended biological specimens such as human embryos. The staining of actin together with chromatin allowed us to specify the architecture of the embryo and the appearance of the nucleus. METHODS: F-Actin and chromatin distributions were visualized using laser scanning confocal microscopy in "fresh" and "cryopreserved" human preimplantation embryos obtained by in vitro fertilization. RESULTS: The current study revealed a high rate of multinucleation in arrested or poor-quality embryos (89%, in grade IV embryos). CONCLUSIONS: Confocal microscopy revealed high levels of multinucleated blastomeres, suggesting that the probable cause of arrested development in these embryos was due to multinucleation of blastomeres.

Laser scanning confocal imaging of abnormal or arrested human preimplantation embryos. Publishing Authors By Initials

For similar heterocyclic compounds: heterocyclic compounds, 3-ring: phenanthridines: propidium research abstracts see: heterocyclic compounds: heterocyclic compounds, 3-ring: phenanthridines: propidium research

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Laser scanning confocal imaging of abnormal or arrested human preimplantation embryos. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of assisted reproduction and genetics

VOLUME: 15

Page Numbers: 485-95

Journal Abbreviation: J. Assist. Reprod. Genet.

ISSN: 1058-0468

DAY: 17

MONTH: Sep

YEAR: 1998

Laser scanning confocal imaging of abnormal or arrested human preimplantation embryos. Information

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LANGUAGE: eng

NlmUniqueID: 9206495

Laser scanning confocal imaging of abnormal or arrested human preimplantation embryos. Keywords Mesh Terms:

KEYWORDS: Propidium

MESH TERMS: chemistry

Chemical & Substance for Abstract: Laser scanning confocal imaging of abnormal or arrested human preimplantation embryos. Information

Substance Name: Propidium

Registry Number: 36015-30-2

Grant and Affiliation Information for Laser scanning confocal imaging of abnormal or arrested human preimplantation embryos.

AFFILIATION: Laboratoire de Biologie de la Reproduction et du Développement, Hôpital Edouard Herriot, Lyon, France.

Country: UNITED STATES

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MEDLINETA: J Assist Reprod Genet

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