Laser-induced mixing in microfluidic channels.

Laser-induced mixing in microfluidic channels. Research Abstract Details 

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Laser-induced mixing in microfluidic channels. Abstract Text:

Amy N Hellman,Kaustubh R Rau,Helen H Yoon,Stephanie Bae,James F Palmer,K Scott Phillips,Nancy L Allbritton,Vasan Venugopalan,

We demonstrate a novel strategy for mixing solutions and initiating chemical reactions in microfluidic systems. This method utilizes highly focused nanosecond laser pulses from a Q-switched Nd:YAG laser at lambda = 532 nm to generate cavitation bubbles within 100- and 200-microm-wide microfluidic channels containing the parallel laminar flow of two fluids. The bubble expansion and subsequent collapse within the channel disrupts the laminar flow of the parallel fluid streams and produces a localized region of mixed fluid. We use time-resolved imaging and fluorescence detection methods to visualize the mixing process and to estimate both the volume of mixed fluid and the time scale for the re-establishment of laminar flow. The results show that mixing is initiated by liquid jets that form upon cavitation bubble collapse and occurs approximately 20 micros following the delivery of the laser pulse. The images also reveal that mixing occurs on the millisecond time scale and that laminar flow is re-established on a 50-ms time scale. This process results in a locally mixed fluid volume in the range of 0.5-1.5 nL that is convected downstream with the main flow in the microchannel. We demonstrate the use of this mixing technique by initiating the horseradish peroxidase-catalyzed reaction between hydrogen peroxide and nonfluorescent N-acetyl-3,7-dihydroxyphenoxazine (Amplex Red) to yield fluorescent resorufin. This approach to generate the mixing of adjacent fluids may prove advantageous in many microfluidic applications as it requires neither tailored channel geometries nor the fabrication of specialized on-chip instrumentation.

Laser-induced mixing in microfluidic channels. Publishing Authors By Initials

AN Hellman,KR Rau,HH Yoon,S Bae,JF Palmer,KS Phillips,NL Allbritton,V Venugopalan,

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: oxazines research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: oxazines research

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Laser-induced mixing in microfluidic channels. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 4484-92

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 18

MONTH: 05

YEAR: 2007

Laser-induced mixing in microfluidic channels. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Laser-induced mixing in microfluidic channels. Keywords Mesh Terms:

KEYWORDS: Oxazines

MESH TERMS: chemistry

Chemical & Substance for Abstract: Laser-induced mixing in microfluidic channels. Information

Substance Name: Horseradish Peroxidase

Registry Number: EC 1.11.1.-

Grant and Affiliation Information for Laser-induced mixing in microfluidic channels.

AFFILIATION: Department of Chemical Engineering and Materials Science, University of California, Irvine, California 92697, USA.

Country: United States

AGENCY: United States NIBIB

GRANT: R01-EB04436

ACRONYM: EB

MEDLINETA: Anal Chem

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