Large-scale purification and characterization of human parathyroid hormone-1 receptor stably expressed in HEK293S GnTI- cells.

Large-scale purification and characterization of human parathyroid hormone-1 receptor stably expressed in HEK293S GnTI- cells. Research Abstract Details 

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Large-scale purification and characterization of human parathyroid hormone-1 receptor stably expressed in HEK293S GnTI- cells. Abstract Text:

Lu Gan,Joseph M Alexander,Angela Wittelsberger,Beena Thomas,Michael Rosenblatt,

Human parathyroid hormone-1 receptor (hPTHR1) belongs to class II of the G protein-coupled receptor (GPCR) family, whose members all contain a seven-transmembrane helix domain. The receptor regulates bone metabolism through interactions with its ligand, human parathyroid hormone (hPTH). For structural studies of the hPTHR1/hPTH complex, we constructed a mammalian cell line to stably express recombinant hPTHR1 in large-scale. The receptor was solubilized with dodecyl maltoside and purified with affinity chromatography. The purified receptor displayed restricted N-glycosylation as expected. Functionality was demonstrated: the hPTHR1 retained affinity for bPTH-(1-34) and specifically cross-linked to a radioiodinated bPTH-(1-34) analog. This work describes an approach for preparing milligram-scale quantities of receptor for elucidation of the structural biology of this seven-transmembrane GPCR.

Large-scale purification and characterization of human parathyroid hormone-1 receptor stably expressed in HEK293S GnTI- cells. Publishing Authors By Initials

L Gan,JM Alexander,A Wittelsberger,B Thomas,M Rosenblatt,

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

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Large-scale purification and characterization of human parathyroid hormone-1 receptor stably expressed in HEK293S GnTI- cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Protein expression and purification

VOLUME: 47

Page Numbers: 296-302

Journal Abbreviation: Protein Expr. Purif.

ISSN: 1046-5928

DAY: 5

MONTH: 12

YEAR: 2005

Large-scale purification and characterization of human parathyroid hormone-1 receptor stably expressed in HEK293S GnTI- cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9101496

Large-scale purification and characterization of human parathyroid hormone-1 receptor stably expressed in HEK293S GnTI- cells. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Large-scale purification and characterization of human parathyroid hormone-1 receptor stably expressed in HEK293S GnTI- cells. Information

Substance Name: Recombinant Proteins

Registry Number: 0

Grant and Affiliation Information for Large-scale purification and characterization of human parathyroid hormone-1 receptor stably expressed in HEK293S GnTI- cells.

AFFILIATION: Department of Physiology, Tufts University, School of Medicine, 136 Harrison Avenue, Boston, MA 02111-1800, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK-47940

ACRONYM: DK

MEDLINETA: Protein Expr Purif

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