Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor.

Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor. Research Abstract Details 

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Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor. Abstract Text:

Andrew T Bauman,Martina Ralle,Ninian J Blackburn,

Rat PHM (peptidylglycine alpha-hydroxylating monooxygenase; EC 1.14.17.3) expressed in CHO DG44 cells as a recombinant protein (rat PHMcc, residues 42-356 cloned in the pCIS vector, A.S. Kolhekar, H.T. Keutman, R. E. Mains, A.S.W. Quon, B.A. Eipper, Biochemistry 36 (1997) 10901-10909), was produced in two different bioreactors, a Cellmax 100 (B1) and an Accusyst-MiniMax (B2). B2 contains features not present in B1, which contribute to environmental control, and ease of operation, and was more successful at producing high quality PHM than B1 in both yield (B1: 5mg/day, B2: 12-15 mg/day), activity (B1: 12-20 micromol O(2)/min/mg, B2: 24-36 micromol O(2)/min/mg), and viability (B1: <6 months, B2: indefinite). Additionally, B1 exhibited clipping at Ser 61, and a decline in quality late in the run. PHM from B2 was of consistent quality and homogeneity throughout the run. The increased yield and purity made possible collection of visible spectra of the Cu(II) sites, and mass spectrometric data not previously available.

Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor. Publishing Authors By Initials

AT Bauman,M Ralle,NJ Blackburn,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research

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Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Protein expression and purification

VOLUME: 51

Page Numbers: 34-8

Journal Abbreviation: Protein Expr. Purif.

ISSN: 1046-5928

DAY: 27

MONTH: 06

YEAR: 2006

Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9101496

Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor. Keywords Mesh Terms:

KEYWORDS: Rats

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor. Information

Substance Name: peptidylglycine monooxygenase

Registry Number: EC 1.14.17.3

Grant and Affiliation Information for Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor.

AFFILIATION: Department of Environmental and Biomolecular Systems, OGI School of Science and Engineering, Oregon Health and Sciences University, Beaverton, OR 97006, USA.

Country: United States

AGENCY: United States PHS

GRANT: NW27583

ACRONYM:

MEDLINETA: Protein Expr Purif

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