Large scale isolation and some properties of AGY-specific serine tRNA from bovine heart mitochondria.

Large scale isolation and some properties of AGY-specific serine tRNA from bovine heart mitochondria. Research Abstract Details 

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Large scale isolation and some properties of AGY-specific serine tRNA from bovine heart mitochondria. Abstract Text:

T Ueda,T Ohta,K Watanabe,

A method was developed for large scale isolation of AGY-specific serine tRNA (tRNASerAGY) from bovine heart mitochondria. By this method, 5 A260 units of tRNASerAGY were recovered from 6.3 kg of bovine hearts. The nucleotide sequence was identical to that reported previously. tRNASerAGY showed abnormal melting profiles, as was predicted from its unique primary sequence. Its secondary and/or tertiary structure was analyzed by nuclease digestion method. It was suggested that three extra base pairs could occur in the anticodon stem region, with one adenosine residue protruding. The T loop was quite sensitive to nuclease S1, suggesting that the T loop doesn't interact with other regions. This finding is consistent with the model proposed by Sundaralingam (1980). tRNASerAGY was aminoacylated in vitro with only mitochondrial enzyme but not with the enzymes from E. coli and yeast. The aminoacylation rate of tRNASerAGY with mitochondrial enzyme was much faster than that of cytosolic tRNASerUCN, perhaps reflecting differences due to the presence and absence of the D arm of the tRNAs.

Large scale isolation and some properties of AGY-specific serine tRNA from bovine heart mitochondria. Publishing Authors By Initials

T Ueda,T Ohta,K Watanabe,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

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Large scale isolation and some properties of AGY-specific serine tRNA from bovine heart mitochondria. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 98

Page Numbers: 1275-84

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1985

Large scale isolation and some properties of AGY-specific serine tRNA from bovine heart mitochondria. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Large scale isolation and some properties of AGY-specific serine tRNA from bovine heart mitochondria. Keywords Mesh Terms:

KEYWORDS: Temperature

MESH TERMS: analysis

Chemical & Substance for Abstract: Large scale isolation and some properties of AGY-specific serine tRNA from bovine heart mitochondria. Information

Substance Name: tRNA, serine-

Registry Number: 0

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Country: JAPAN

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MEDLINETA: J Biochem

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