Large-scale analysis of 73 329 physcomitrella plants transformed with different gene disruption libraries: production parameters and mutant phenotypes.

Large-scale analysis of 73 329 physcomitrella plants transformed with different gene disruption libraries: production parameters and mutant phenotypes. Research Abstract Details 

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Large-scale analysis of 73 329 physcomitrella plants transformed with different gene disruption libraries: production parameters and mutant phenotypes. Abstract Text:

G Schween,T Egener,D Fritzowsky,J Granado,M-C Guitton,N Hartmann,A Hohe,H Holtorf,D Lang,J M Lucht,C Reinhard,S A Rensing,K Schlink,J Schulte,R Reski,

Gene targeting in the moss Physcomitrella patens has created a new platform for plant functional genomics. We produced a mutant collection of 73 329 Physcomitrella plants and evaluated the phenotype of each transformant in comparison to wild type Physcomitrella. Production parameters and morphological changes in 16 categories, such as plant structure, colour, coverage with gametophores, cell shape, etc., were listed and all data were compiled in a database (mossDB). Our mutant collection consists of at least 1804 auxotrophic mutants which showed growth defects on minimal Knop medium but were rescued on supplemented medium. 8129 haploid and 11 068 polyploid transformants had morphological alterations. 9 % of the haploid transformants had deviations in the leaf shape, 7 % developed less gametophores or had a different leaf cell shape. Other morphological deviations in plant structure, colour, and uniformity of leaves on a moss colony were less frequently observed. Preculture conditions of the plant material and the cDNA library (representing genes from either protonema, gametophore or sporophyte tissue) used to transform Physcomitrella had an effect on the number of transformants per transformation. We found correlations between ploidy level and plant morphology and growth rate on Knop medium. In haploid transformants correlations between the percentage of plants with specific phenotypes and the cDNA library used for transformation were detected. The number of different cDNAs present during transformation had no effect on the number of transformants per transformation, but it had an effect on the overall percentage of plants with phenotypic deviations. We conclude that by linking incoming molecular, proteome, and metabolome data of the transformants in the future, the database mossDB will be a valuable biological resource for systems biology.

Large-scale analysis of 73 329 physcomitrella plants transformed with different gene disruption libraries: production parameters and mutant phenotypes. Publishing Authors By Initials

G Schween,T Egener,D Fritzowsky,J Granado,MC Guitton,N Hartmann,A Hohe,H Holtorf,D Lang,JM Lucht,C Reinhard,SA Rensing,K Schlink,J Schulte,R Reski,

For similar genetic structures: plasmids research abstracts see: genetic structures: plasmids research

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Large-scale analysis of 73 329 physcomitrella plants transformed with different gene disruption libraries: production parameters and mutant phenotypes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Plant biology (Stuttgart, Germany)

VOLUME: 7

Page Numbers: 228-37

Journal Abbreviation: Plant Biol (Stuttg)

ISSN: 1435-8603

DAY: 7

MONTH: May

YEAR: 2005

Large-scale analysis of 73 329 physcomitrella plants transformed with different gene disruption libraries: production parameters and mutant phenotypes. Information

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LANGUAGE: eng

NlmUniqueID: 101148926

Large-scale analysis of 73 329 physcomitrella plants transformed with different gene disruption libraries: production parameters and mutant phenotypes. Keywords Mesh Terms:

KEYWORDS: Plasmids

MESH TERMS: genetics

Chemical & Substance for Abstract: Large-scale analysis of 73 329 physcomitrella plants transformed with different gene disruption libraries: production parameters and mutant phenotypes. Information

Substance Name: DNA, Plant

Registry Number: 0

Grant and Affiliation Information for Large-scale analysis of 73 329 physcomitrella plants transformed with different gene disruption libraries: production parameters and mutant phenotypes.

AFFILIATION: Plant Biotechnology, Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.

Country: Germany

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MEDLINETA: Plant Biol (Stuttg)

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