Lack of thrombospondin-1 increases angiogenesis in a model of chronic inflammatory bowel disease.

Lack of thrombospondin-1 increases angiogenesis in a model of chronic inflammatory bowel disease. Research Abstract Details 

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Lack of thrombospondin-1 increases angiogenesis in a model of chronic inflammatory bowel disease. Abstract Text:

Samantha Zak,John Treven,Nolly Nash,Linda S Gutierrez,

BACKGROUND AND AIMS: Vascular abnormalities and expression of pro-angiogenic factors are observed in inflammatory bowel diseases (IBD). In this study, the role of thrombospondin-1 (TSP-1), an antiangiogenic protein, was analyzed using the dextran sulfate sodium (DSS) model for IBD. MATERIALS AND METHODS: Wild-type (WT) and thrombospondin-1-deficient (TSP-1(-/-)) mice were subjected to four cycles (7 days) of DSS. Basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), transforming growth factor beta 1 (TGFss-1), and pro-apoptotic proteins such as Fas and its ligand (FasL) were determined by enzyme-linked immunosorbent assay. Double immunohistochemistry for cluster of differential 31 (CD31) and panendothelial cell antigen-32 antibodies was performed for detecting blood vessels. The terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay was also performed for identifying apoptotic cells. Inflammation, dysplasia, microvascular density (MVD), apoptotic indices (AI), protein 53 (p53), and ss-catenin expression were determined. RESULTS: VEGF and bFGF protein levels and MVD were higher in the TSP-1(-/-) mice (p = 0.0312, p = 0.0246, and p = 0.0085, respectively). AI in the endothelial cells (EC) and FasL levels were significantly lower in TSP-1(-/-) compared to WT mice (p = 0.0042 and p = 0.0362, respectively). Dysplasia was detected in 66% of TSP-1(-/-) mice compared to 14% in WT mice. Hscores of ss-catenin and areas overexpressing p53 were higher in TSP-1(-/-) mice (p = 0.0002 and p = 0.0339, respectively). CONCLUSION: TSP-1 may decrease angiogenesis by reducing the levels of pro-angiogenic factors and inducing apoptosis in EC through the Fas or FasL pathway. These findings, along with the increased overexpression of p53 and ss-catenin in TSP-1(-/-) mice, underline its role in carcinogenesis.

Lack of thrombospondin-1 increases angiogenesis in a model of chronic inflammatory bowel disease. Publishing Authors By Initials

S Zak,J Treven,N Nash,LS Gutierrez,

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Lack of thrombospondin-1 increases angiogenesis in a model of chronic inflammatory bowel disease. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: International journal of colorectal disease

VOLUME: 23

Page Numbers: 297-304

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ISSN: 0179-1958

DAY: 28

MONTH: 11

YEAR: 2007

Lack of thrombospondin-1 increases angiogenesis in a model of chronic inflammatory bowel disease. Information

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LANGUAGE: eng

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AFFILIATION: Department of Biology, Wilkes University, 84 W. South St., Wilkes-Barre, PA, USA, linda.gutierrez@wilkes.edu.

Country: Germany

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MEDLINETA: Int J Colorectal Dis

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