Lack of iNOS facilitates MCMV spread in the retina.

Lack of iNOS facilitates MCMV spread in the retina. Research Abstract Details 

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Lack of iNOS facilitates MCMV spread in the retina. Abstract Text:

Ming Zhang,Jun Zhou,Brendan Marshall,Hua Xin,Sally S Atherton,

PURPOSE: The purposes of this study were to identify iNOS-producing retinal cells and to determine whether lack of iNOS facilitates MCMV spread and replication in the retina. METHODS: Immunosuppressed (IS) iNOS(-/-) mice or C57BL/6 (wild-type) mice were inoculated with 5 x 10(4) PFU of MCMV K181 strain (K181) via the supraciliary route. Injected eyes were collected at several times after inoculation and examined by plaque assay for replicating virus, RT-PCR for iNOS RNA, Western blot for iNOS protein and by staining for MCMV early antigen (EA), iNOS, and retinal cell antigens. RESULTS: iNOS mRNA and iNOS proteins were expressed in the MCMV-injected eye of wild-type mice. Most iNOS-producing cells were F4/80-positive, including macrophages, RPE-derived macrophages, and resident microglia. Significantly higher titers of virus were recovered from the injected eyes, and more infected cells were detected in the retina of IS iNOS(-/-) mice than in IS wild-type mice. Retinal necrosis and loss of retinal architecture throughout the retina were noted in IS iNOS(-/-) mice, whereas cytomegalic cells and retinitis were present only in the peripheral retina of IS wild-type mice. CONCLUSIONS: iNOS produced by macrophages, especially resident macrophages including microglia and RPE derived macrophages, plays an important role in limiting spread of MCMV in the retina.

Lack of iNOS facilitates MCMV spread in the retina. Publishing Authors By Initials

M Zhang,J Zhou,B Marshall,H Xin,SS Atherton,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research

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MEDLINE DATE:

Lack of iNOS facilitates MCMV spread in the retina. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Investigative ophthalmology & visual science

VOLUME: 48

Page Numbers: 285-92

Journal Abbreviation: Invest. Ophthalmol. Vis. Sci.

ISSN: 0146-0404

DAY: 3

MONTH: Jan

YEAR: 2007

Lack of iNOS facilitates MCMV spread in the retina. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7703701

Lack of iNOS facilitates MCMV spread in the retina. Keywords Mesh Terms:

KEYWORDS: Virus Replication

MESH TERMS: virology

Chemical & Substance for Abstract: Lack of iNOS facilitates MCMV spread in the retina. Information

Substance Name: Nos2 protein, mouse

Registry Number: EC 1.14.13.39

Grant and Affiliation Information for Lack of iNOS facilitates MCMV spread in the retina.

AFFILIATION: Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta, Georgia 30912, USA.

Country: United States

AGENCY: United States NEI

GRANT: EY009169

ACRONYM: EY

MEDLINETA: Invest Ophthalmol Vis Sci

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