Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells.

Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells. Abstract Text:

Oliver Zeitz,Lars Schlichting,Gisbert Richard,Olaf Strauss,Oliver Zeitz,Lars Schlichting,Gisbert Richard,Olaf Strauss,

BACKGROUND: Oxidative damage to the retinal pigment epithelium might be involved in the pathogenesis of age related macular degeneration. Thus antioxidative protection represents a rationale for a causative therapy or prophylaxis. The aim of the present study is to evaluate antioxidative properties of vitamin C and pyruvate at retinal pigment epithelial (RPE) cells exposed to oxidative stress. METHODS: The ability of vitamin C and pyruvate to quench hydroxyl radicals was tested using the di-hydro-rhodamine (DHR) assay. Cells of the human RPE cell line ARPE-19 were exposed for 8 min to hydroxyl radicals generated by the Fenton reaction from 2.25 mM H2O2 and 30 microM Fe3+ -nitrilo-tri-acetate. This was done in the absence and presence of 0.3-3.0 mM pyruvate and vitamin C, respectively. Cell survival was analysed by vitality staining (life-dead-assay) and expressed as cell survival ratio. A survival ratio <1.0 indicates cell loss. RESULTS: At concentrations from 0.1 to 1.0 mM vitamin C and pyruvate quench hydroxyl radicals in the DHR assay in absence of living matter. In the presence of 0.1- 0.3 mM vitamin C and pyruvate, ARPE-19 showed a reduced survival ratio (0.87 +/- 0.01 to 0.89 +/- 0.02 after 6 h) which was not the case at the higher concentrations between 1 and 3 mM. The exposure of ARPE-19 cells to hydroxyl radicals reduced the survival ratio to 0.92 +/- 0.02. At concentrations at which vitamin C and pyruvate exert toxic effects, a potentiation of radical induced cell death can be observed (survival ratio 0.79 +/- 0.02 and 0.82 +/- 0.03, respectively). Higher concentrations of vitamin C or pyruvate had no explicit protective effect to the hydroxyl radical induced damage. DISCUSSION: Although vitamin C and pyruvate are potent hydroxyl radical quenchers in vitro they failed to protect cultured ARPE-19 cells from oxidative stress induced cell death. In contrast, when applying the scavengers at low concentrations a potentiation of cell damage was observed.

Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells. Publishing Authors By Initials

O Zeitz,L Schlichting,G Richard,O Strauss,O Zeitz,L Schlichting,G Richard,O Strauss,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Graefe's archive for clinical and experimental oph

VOLUME: 245

Page Numbers: 276-81

Journal Abbreviation: Graefes Arch. Clin. Exp. Ophth

ISSN: 0721-832X

DAY: 23

MONTH: Feb

YEAR: 2007

Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8205248

Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells.

AFFILIATION: Universitätsklinikum Hamburg-Eppendorf, Klinik und Poliklinik ffir Augenheilkunde, Martinistr. 52, 20246 Hamburg, Germany. zeitz@uke.uni-hamburg.de

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Graefes Arch Clin Exp Ophthalm

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells Related Publications

• A heterozygous mutation disrupting the SPAG16 gene results in biochemical instability of central apparatus components of the human sperm axoneme.
• Accelerated mortality from hydrocephalus and pneumonia in mice with a combined deficiency of SPAG6 and SPAG16L reveals a functional interrelationship between the two central apparatus proteins.
• Adaptive time-scale feature extraction in electroencephalographic responses to transcranial magnetic stimulation.
• Cadophora finlandia and Phialocephala fortinii: Agrobacterium-mediated transformation and functional GFP expression.
• Changes in histological criteria lead to different prevalences of nonalcoholic steatohepatitis in severe obesity.
• Denial of the climacteric -- a pilot study of a common clinical phenomenon.
• Development of a nurse practice arrangement in rural Appalachia: triumphs and challenges.
• Discovering and verifying elusive fullerene cage isomers: structures of C2-p11-(C74-D3h)(CF3)12 and C2-p11-(C(78)-D3h5)(CF3)12.
• Flexible endoscopic nasopharyngoscopy.
• Gender differences in traumatic events and rates of post-traumatic stress disorder among homeless youth.
• Genetic approaches to polycystic ovarian syndrome.
• Genetics of polycystic ovarian syndrome.
• Helminth eggs inactivation efficiency by faecal sludge dewatering and co-composting in tropical climates.
• Issues for DSM-V: incorporating patients' subjective experiences.
• Lack of antioxidative properties of vitamin C and pyruvate in cultured retinal pigment epithelial cells.
• Large-scale inverse and forward modeling of adaptive resonance in the tinnitus decompensation.
• Modeling Neural Correlates of Auditory Attention in Evoked Potentials using Corticothalamic Feedback Dynamics.
• Molecular abnormalities in oocytes from women with polycystic ovary syndrome revealed by microarray analysis.
• Non-injection drug use and Hepatitis C Virus: a systematic review.
• Nuclear medicine applications in molecular imaging: 2007 update.
• Possible macroscopic indicators of neural maturation in subcortical auditory pathways in school¿age children.
• Radionuclide techniques for identifying vulnerable plaque.
• Role of active site tyrosines in dynamic aspects of DNA binding by AP endonuclease.
• Segmentation of neck lymph nodes in CT datasets with stable 3D mass-spring models segmentation of neck lymph nodes.
• Self-associated, "distillable" ionic media.
• Somaclonal Variation of Scopolamine Content in Protoplast-Derived Cell Culture Clones of Hyoscyamus muticus.
• Synthesis and structure of Ag(1-Me-12-SiPh3-CB11F10): selective F12 substitution in 1-Me-CB11F11- and the first Ag(arene)4+ tetrahedron.
• The next 20 years: how customer and workforce attitudes will evolve.
• The role of attention in the tinnitus decompensation: reinforcement of a large-scale neural decompensation measure.
• Thermodynamics of hydrophobic polyacids.