Label-free electrochemical detection of DNA using ferrocene-containing cationic polythiophene and PNA probes on nanogold modified electrodes.

Label-free electrochemical detection of DNA using ferrocene-containing cationic polythiophene and PNA probes on nanogold modified electrodes. Research Abstract Details 

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Label-free electrochemical detection of DNA using ferrocene-containing cationic polythiophene and PNA probes on nanogold modified electrodes. Abstract Text:

Bin Fang,Shoufeng Jiao,Maoguo Li,Yuan Qu,Ximing Jiang,Bin Fang,Shoufeng Jiao,Maoguo Li,Yuan Qu,Ximing Jiang,

A label-free electrochemical method for the detection of DNA-PNA hybridization using a water-soluble, ferrocene-functionalized polythiophene transducer and single-stranded PNA probes on the nanogold modified electrode is investigated. Nanogold modified electrodes can largely increase the immobilization amount of ss-PNA capture probe and lead to an increase of the electrical signal. The ferrocene-containing cationic polythiophene do not interact electrostatically with the PNA probes due to the absence of the anionic phosphate groups on the PNA probes. But after DNA-PNA hybridization, cationic polythiophene is adsorbed on the DNA backbone, giving a clear hybridization detection signal in differential pulse voltammetry (DPV). Very good discrimination against non-complementary DNA and four-base mismatch DNA is observed. These studies show that the proposed method can provide an alternative for expanding the range of detection methods available for DNA hybridization.

Label-free electrochemical detection of DNA using ferrocene-containing cationic polythiophene and PNA probes on nanogold modified electrodes. Publishing Authors By Initials

B Fang,S Jiao,M Li,Y Qu,X Jiang,B Fang,S Jiao,M Li,Y Qu,X Jiang,

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Label-free electrochemical detection of DNA using ferrocene-containing cationic polythiophene and PNA probes on nanogold modified electrodes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biosensors & bioelectronics

VOLUME: 23

Page Numbers: 1175-9

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ISSN: 0956-5663

DAY: 4

MONTH: 11

YEAR: 2007

Label-free electrochemical detection of DNA using ferrocene-containing cationic polythiophene and PNA probes on nanogold modified electrodes. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Label-free electrochemical detection of DNA using ferrocene-containing cationic polythiophene and PNA probes on nanogold modified electrodes.

AFFILIATION: College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China; Anhui Key Laboratory of Chemo-Biosensing, Wuhu 241000, China.

Country: England

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MEDLINETA: Biosens Bioelectron

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