L-Lysine:2-oxoglutarate 6-aminotransferase from Flavobacterium lutescence (= Achromobacter liquidum) has been shown to be composed of one each of four non-identical subunits, A, B1, B2, and C. The subunits were isolated by gel filtration, and DEAE-cellulose chromatography in the presence of 8 M urea. Their molecular weights were determined by ultracentrifugation, gel electrophoresis and gel filtration: subunit A 24,000; B1 28,000; B2 28,000; C 45,000. These subunits were all different in amino acid composition. Of the two molecules of bound pyridoxal 5'-phosphate, the one which absorbs at 415 nm is bound to subunit B2 and participates in the catalytic action of the enzyme.
L-Lysine: 2-oxoglutarate 6-aminotransferase. Subunit structure composed of non-identical polypeptides and pyridoxal 5'-phosphate-binding subunit. Publishing Authors By Initials
L-Lysine: 2-oxoglutarate 6-aminotransferase. Subunit structure composed of non-identical polypeptides and pyridoxal 5'-phosphate-binding subunit. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Journal of biochemistry
VOLUME: 87
Page Numbers: 1395-402
Journal Abbreviation: J. Biochem.
ISSN: 0021-924X
DAY: 19
MONTH: May
YEAR: 1980
L-Lysine: 2-oxoglutarate 6-aminotransferase. Subunit structure composed of non-identical polypeptides and pyridoxal 5'-phosphate-binding subunit. Information
Number of References:
LANGUAGE: eng
NlmUniqueID: 376600
L-Lysine: 2-oxoglutarate 6-aminotransferase. Subunit structure composed of non-identical polypeptides and pyridoxal 5'-phosphate-binding subunit. Keywords Mesh Terms:
KEYWORDS: Transaminases
MESH TERMS: metabolism
Chemical & Substance for Abstract: L-Lysine: 2-oxoglutarate 6-aminotransferase. Subunit structure composed of non-identical polypeptides and pyridoxal 5'-phosphate-binding subunit. Information
Substance Name: L-Lysine 6-Transaminase
Registry Number: EC 2.6.1.36
Grant and Affiliation Information for L-Lysine: 2-oxoglutarate 6-aminotransferase. Subunit structure composed of non-identical polypeptides and pyridoxal 5'-phosphate-binding subunit.
AFFILIATION:
Country: JAPAN
AGENCY:
GRANT:
ACRONYM:
MEDLINETA: J Biochem
REFSOURCE:
DATABASENAME:
ACCESSION NUMBER:
Number Hits: 0
L-Lysine: 2-oxoglutarate 6-aminotransferase Subunit structure composed of non-identical polypeptides and pyridoxal 5'-phosphate-binding subunit Related Publications